Skip to main content
SpringerLink
Log in

A Kinetic Model of the Synergism of Endo- and Exoglucanase and β-Glucosidase on Hydrolysis of Cellulose

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A kinetic model representing the synergistic action of the three components that compose cellulase on hydrolysis of solid cellulose particles is proposed.

The model consists of three simultaneous differential equations: one representing the action of the endoenzyme, another representing the action of the exoenzyme, and the third representing the action of the β-glucosidase. A simultaneous solution of these three equations expresses the synergism.

The experimental data fit the theory well.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

[C]:

Concentration of cellobiose mol/mL

[Een]:

Concentration of EG U/mL

[Eenf]:

Concentration of free EG U/mL

[G]:

Concentration of glucose mol/mL

[C.Een]:

Concentration of EG-cellobiose complex mol/mL

ken :

Reaction rate constant of EG mg/min U

te]Ki1 :

Inhibition constant of cellobiose, Eqs. 1 and 3 mol/mL

K13 :

Inhibition constant of glucose, Eq. 5 mol/mL

Kb :

Inhibition constant of glucose, Eq. 6 mol/mL

Ki2 :

Inhibition constant of cellobiose for EG mol/mL

Ki4 :

Inhibition constant of glucose for EG mol/mL

Km :

M-M constant of CBH mg/mL

Kmb :

M-M constant of BG mol/mL

[S]:

Concentration of the substrate mg/mL

[S0]:

Initial concentration of the substrate mg/mL

t:

Reaction time min

Vm :

Maximum velocity of CBH mol/mL min

Vmb :

Maximum velocity of BG mol/mL min

References

  1. Halliwell, G. and Griffin, M. (1973),Biochem. J. 135, 587–594.

    CAS  Google Scholar 

  2. Wood, T. M. and McCrae, S. I. (1979),Hydrolysis of Cellulose: Mechanisms of Enzymatic and Acid Catalysis, Brown, R. D. and Jurasek, L., eds., American Chemical Society, pp. 181-209.

  3. Wood, T. M. (1988),Methods Enzymol. 160 (Biomass, PtA.), 221–233.

    Article  CAS  Google Scholar 

  4. Woodward, J., Lima, M., Lee, N. E. (1988),Biochem. J. 255, 895–899.

    CAS  Google Scholar 

  5. Wood, T. M., McCrae, S. I., and Bhat, K. M. (1989), The mechanism of fungal cellulase action.Biochem. J. 260, 37–43.

    CAS  Google Scholar 

  6. Fujii, M., Homma, T., Shimizu, M., and Taniguchi, M. (1988),Recent Advances in Biotechnology and Applied Biology, Chang, S., Chan, K., Wood, N. Y. S., eds., Chinese University Press, Hong Kong, 521–530.

    Google Scholar 

  7. Beldman, G., Voragen, A. G. J., Rombouts, F. M., Seale-vanLeeuwen, M. F., and Pilnik, W. (1987),Biotechnol. Bioeng. 30, 251–257.

    Article  CAS  Google Scholar 

  8. Somogyi, M. (1952),J. Biol. Chem. 195, 19–23.

    CAS  Google Scholar 

  9. Taniguchi, M., Tanaka, M., Matsuno, R., and Kamikubo, T. (1980),J. Ferment. Technol. 58, 143–148.

    CAS  Google Scholar 

  10. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265–275.

    CAS  Google Scholar 

  11. Okuda, J. and Miwa, I. (1971),Anal. Biochem. 39, 387–394.

    Article  CAS  Google Scholar 

  12. Okuda, J. and Miwa, I. (1973),Methods Biochem. Anal. 21, 155–189.

    Article  CAS  Google Scholar 

  13. Lineweaver, H. and Burk, D. (1934),J. Am. Chem. Soc. 56, 658–666.

    Article  CAS  Google Scholar 

  14. Dixon, M. (1953),Biochem. J. 55, 170–171.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Nügata University, Ikarashi 2, 950-21, Nügata, Japan

    Michihiro Fujii, Taira Homma, Kazuhisa Ooshima & Masayuki Taniguchi

Authors
  1. Michihiro Fujii
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Taira Homma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuhisa Ooshima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masayuki Taniguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujii, M., Homma, T., Ooshima, K. et al. A Kinetic Model of the Synergism of Endo- and Exoglucanase and β-Glucosidase on Hydrolysis of Cellulose. Appl Biochem Biotechnol 28, 145–156 (1991). https://doi.org/10.1007/BF02922596

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02922596

Index Entries

Search

Navigation