Abstract
Water stress in the leaves was induced by gradual decreasing of substrate moisture in five-day cycles.The hydration level of the leaves was characterized by their water potential (ψw), osmotic potential (ψs), pressure potential ψp and water saturation deficit(ΔW sat ).The activities of Photosystems 1 and 2 were determined polarographically with Pt/Ag(AgOH) electrode as changes in oxygen concentration in chloroplast suspensions. The shape of light curves of Hill reaction was not influenced by leaf (ψw), hence both quantum efficiency and dark phase of this process were affected in a similar manner by water deficit. The activities of both photosystems measured at saturating photon flux density declined with the lowering of leaf (ψw) (in the range from -5 to -14 x 105 Pa) and the decrease in activity of Photosystem 2 was more rapid than that of Photosystem 1. The ratio of activities of Photosystems 1 and 2 was mildly enhanced by a lowering of (ψw), but it decreased with increasing age. The lowering of ψwinduced lowering in the chlorophyll a/b ratio thus concealing the usual ontogenetic course of this ratio.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartoš, J., Berková, E., Šetlík, I.: A versatile chamber for gas exchange measurements in suspensions of algae and chloroplasts. - Photosynthetica9: 395–406, 1975.
Boyer, J. S., Bowen, B. L.: Inhibition of oxygen evolution in chloroplasts isolated from leaves with low water potentials. - Plant Physiol.45: 612–615, 1970.
Boyek, J. S., Potter, J. R.: Chloroplast response to low leaf water potentials. I. Role of turgor. Plant Physiol.51: 989–992, 1973.
Fry, K. E.: Some factors affecting the Hill reaction activity in cotton ehloroplasts. - Plant Physiol.45: 465–469, 1970.
Fky, K. E.: Inhibition of ferricyanide reduction in chloroplasts prepared from water-stressed cotton leaves. - Crop Sci.12: 698–701, 1972.
Keck, R. W., Boyer, J. S.: Chloroplast response to low leaf water potentials. III. Differing inhibition of electron transport and photophosphorylation. - Plant Physiol.53: 474–479, 1974.
Mohanty, P., Boyer, J. S.: Quantum yield of photosynthesis in leaves and chloroplasts when the leaves have low water potentials. - Plant Physiol.56 (Suppl.): 61, 1975.
Platjt, Z.: Inhibition of photosynthetic carbon dioxide fixation in isolated spinach chloroplasts exposed to reduced osmotic potentials. - Plant Physiol.48: 591–595, 1971.
Pospíšilová, J.: Water potential-water saturation deficit relationship during dehydration and resaturation of leaves. - Biol. Plant.15: 290–293, 1973.
Potter, J. R., Boyer, J. S.: Chloroplast response to low leaf water potentials. II. Role of osmotic potential. - Plant Physiol.51: 993–997, 1973.
Santarius, K. A.: Das Verhalten von CO2-Assimilation,NADP- und PGS-Reduktion und ATP- -Synthese intakter Blattzellen in Abhängigkeit vom Wassergehalt. - Planta73: 228–242, 1967.
Santarius, K. A., Ernst, R.: Das Verhalten von Hill-Reaktion und Photophosphorylierung isolierter Chloroplasten in Abhängigkeit vom Wassergohalt. I. Wasserentzug mittels kon -zentrierter Lösungen. - Planta73: 91–108, 1967.
Santarius, K. A., Heber, U.: Das Verhalten von Hill-Reaktion und Photophosphorylierung isolierter Chloroplasten in Abhangigkeit vom Wassergehalt. II. Wasserentzug über CaCl2. - Planta73: 109–137, 1967.
Šesták, Z.: Determination of chlorophyllsa andb. - In:Šesták, Z., Čatský, J., JArvis, P. G. (ed.): Plant Photosynthetic Production. Manual of Methods. Pp. 672–701. Dr. W. Junk N. V. Publ., The Hague 1971.
Šesták, Z., Čatský, J.: Sur les relations entro le contenu en chlorophylle ot l’activité photo- synthétique pendant la croissance et le vieillissement des feuilles. - In:Sironval, C. (ed.): Le Chloroplaste, Croissance et Vieillissement. Pp. 213–262. Masson et Cie, Paris 1967.
Šesták, Z., Čatský, J., Solábová, J., Strnhadová, H., Tichá, I.: Carbon dioxide transfer and photochemical activities as factors of photosynthesis during ontogenosis of primary bean leaves. - In:Nasybov, YTT. S., Šesták, Z. (ed.): Genetic Aspects of Photosynthesis. Pp. 159 to 166. Dr. W. Junk B. V. Publ., The Hague1975.
Vieira-Da-Silva, J. B., Veltkamt, J.: Action du potentiel osmotique de la solution nutritive sur la réaction de Hill et la phosphorylation de chloroplastes de Cotonnier. - Compt. rend. Acad. Sci. Paris, Ser. D271: 1367–1379, 1970.
Crow, J. F., Kimura, M.: An Introduction to Population Genetics Theory. - Harper & Row, Publ., New York 1970.
Květ, J., Marshall, J. K.: Assessment of leaf area and other assimilating plant surfaces. -In:Šesták, Z., Čatský, J., JARVIS, P. G. (ed.): Plant Photosynthetie Production. Manual of Methods. Pp. 517–555. Dr. W. Junk, Publ. The Hague 1971.
Morrow, P. A., Slatyer, R. O.: Leaf temperature effects on measurements of diffusive resistance to water vapor transfer. - Plant Physiol.47: 559–561, 1971.
Abbreviations of journals names, and transliteration of Cyrillic characters should follow the “Current Contents”. Unpublished data, personal communications and articles in preparation should be referred to parenthetically in the text only.
In the text, only two authors are cited: DALTON and FELIX (1956) or (DALTOH and FELIX 1956). With more authors, use MOHRet at}. (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PospíŠilová, J., Zima, J. & Šesták, Z. Effect of hydration level in primary bean leaves on the activity of photosystems 1 and 2 in isolated chloroplasts. Biol Plant 18, 473–482 (1976). https://doi.org/10.1007/BF02922558
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02922558