Abstract
Mitochondria from dwarf and tall light-grown peas have been separated from contaminating chloroplasts and other organelles on discontinuous sucrose gradients. A comparison between activities of the mitochondria from the two cultivars of light-grown pea seedlings was made. It was observed that both respiratory control (R.C.) indices and phosphate to oxygen (P : O) ratios were superior in mitochondria from the tall cultivar than the dwarf cultivar. The results are discussed in relation to energy status and possible gibberellic acid content in the two cultivars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chance, B., Williams, G. R.: A simple and rapid assay of oxidative phosphorylation. — Nature175: 1120, 1955.
Douce, R., Christensen, E. L., Bonnek, W. D. Jr.: Preparation of intact plant mitochondria. — Biochim. biophys. Acta275 : 148–100, 1972.
Gornall, A. G., Baradawill, G. J., David, M. M.: Determination of serum proteins by means of the Biuret reaction. — J. biol. Chem.177: 751–766, 1949.
Iseaelstam, G. F., Fukumoto, K.: Phosphorylation and respiration of dwarf and normal pea mitochondria and respiration of internodal sections. — Can. J. Bot.52: 845–848, 1974.
Lehninger, A. L.: The Mitochondrion. — Benjamin, New York 1964.
Mashtakov, S. M., Voinilo, V. A., Deeva, V. P.: Change of oxidative phosphorylation and structure of the respiratory chain of pea mitochondria under the effect of various physiologically active compounds. (English translation). — Fiziol. Rast.16: 91–97, 1969.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Israelstam, G.F., Fukumoto, K. Activity of mitochondria isolated and purified from dwarf and tall, light-grown pea seedlings. Biol Plant 19, 53–58 (1977). https://doi.org/10.1007/BF02922487
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02922487