Skip to main content
Log in

The specificity of the effect of 2,4-D and NAA of the growth, micromorphology, and occurrence of starch in long-termNicotiana tabacum L. Cell strains

  • Published:
Biologia Plantarum

Abstract

The characteristic micromorphology of the tobaoco cell strains, or its cyclic changes in the course of the subcultivation interval can be affected by auxin composition of the medium,i.e. by the application of either 2,4-D alone, or NAA, or their combination. On omitting one of the auxins, the over-all growth of the cultures is not substantially affeoted; however, the participation of various oell types, as well as the occurrenoe of starch grains are altered. The presenoe of 2,4-D alone results in an inhibition of starch occurrence, NAA alone causes a stimulation. There is no causal dependence of the occurrence or absence of starch grains on the stimulation of elongation (volume) growth, or, on the contrary, on cell division.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Brossard, D.: Évolution de la synthèse d’amidon durant l’organisation des nodules puis desméristèmes primaires obtenusin vitro, à partir d’explants de moelle de tabac. Comparaisonavec le point végétatif de la plante entière. — Compt. rend. Acad. Soi. (Paris) Sér. D271: 66 to 59, 1970.

    Google Scholar 

  • Davey, M. R., Fowler, M. W., Street, H. E.: Cell clones oontroled in growth, morphology andpigmentation isolated from a callus culture ofAtropa belladonna var.lutea. — Phytochemistry10: 2559–2575, 1971.

    Article  CAS  Google Scholar 

  • Gautheret, R. J.: La culture des tissus végétaux; techniques et réalisationa. — Masson, Paris 1959.

    Google Scholar 

  • Luštinec, J., Kamínek, M., HadačOvá, V.: Hormonal control of staroh accumulation and cellexpansion in tobacco stem pith. — Proc. IV Symp. Plant Growth Regulators, Toruń 1974.

    Google Scholar 

  • Miller, C. A.: Control of deoxyisofiavone synthesis in soybean tissue. — Planta87: 26–35, 1969.

    Article  CAS  PubMed  Google Scholar 

  • OpatrnÝ, Z.: [Plant tissue cultures in genetics.] In Czech. — Diss. Thesis, Czech. Aoad. Soi.Praha 1971.

  • OpatrnÝ, Z., OpatrnÁ, J.: The influence of growth substances (auxins and cytokinins) on thestaroh synthesis and morphogenesis of two cell strains ofNicotiana tabacum L. — Proc. Conf.Natural Growth Substances, Liblice 1972.

  • Ross, M. K., Thorpe, T. A.: Physiological gradients and shoot initiation in tobaooo callusoultures. — Plant Cell Physiol.14: 473–480, 1973.

    CAS  Google Scholar 

  • Ross, M. K., Thorpe, T. A., Costerton, J. W.: Ultrastructural aspects of shoot initiation intobacco callus oultures. — Amer. J. Bot.60: 788–796, 1973.

    Article  Google Scholar 

  • Thorpe, T. A., Meier, D. D.: Starch metabolism, respiration and shoot formation in tobaccocallus oultures. — Physiol. Plant.27: 365–369, 1972.

    Article  CAS  Google Scholar 

  • Thorpe, T. A., Murashige, T.: Staroh accumulation in shoot-forming tobacco oallus cultures. —Scienoe160: 421–422, 1968.

    Article  CAS  Google Scholar 

  • Thorpe, T. A., Murashige, T.: Some histoohemical changes underlying shoot initiation in tobaccocallus oultures. — Can. J. Bot.48: 277–285, 1970.

    Article  CAS  Google Scholar 

  • Usciatti, M., Codaccioni, M., Guern, J.: Early cytologioal and biochemical events induced bya 6-benzylaminopurine application on inhibited axillary buds ofCicer arietinum plants. —J. exp. Bot.23: 1009–1020, 1972.

    Article  Google Scholar 

  • White, P. R.: A Handbook of Plant Tissue Culture. — Jacques Cattell Press, Lancaster, Pensyl-vania 1943.

    Google Scholar 

  • Witham, F. H.: Effect of 2,4-dichlorphenoxyacetic acid on the cytokinin requirement of soybeancotyledon and tobaooo stem pith callus tissue. — Plant Physiol.43: 1455–1457, 1968.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

OpatrnÝ, Z., OpatbnÁ, J. The specificity of the effect of 2,4-D and NAA of the growth, micromorphology, and occurrence of starch in long-termNicotiana tabacum L. Cell strains. Biol Plant 18, 359–365 (1976). https://doi.org/10.1007/BF02922462

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02922462

Keywords

Navigation