Applied Biochemistry and Biotechnology

, Volume 33, Issue 1, pp 1–14 | Cite as

Supported aqueous-phase enzymatic catalysis in organic media

  • Sanghamitra Parida
  • Ravindra Datta
  • Jonathan S. Dordick


The use of partially hydrated porous silica particles has been studied as a support for cofactor-dependent enzymatic catalysis in organic solvents. At an optimal pore hydration corresponding to 70% pore volume, horse liver alcohol dehydrogenase catalyzes the oxidation and reduction of alcohols and aldehydes, respectively, with rates sixfold higher than with nonporous glass beads as the enzymatic support and with cofactor recycling numbers in excess of 105. Thus, supported aqueous-phase enzymatic catalysis makes highly effective use of the enzyme and cofactor by coimmobilization and by providing a high interfacial area for reactions in organic media.

Index Entries

Supported liquid-phase catalysis alcohol dehydrogenase organic media 


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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Sanghamitra Parida
    • 1
  • Ravindra Datta
    • 1
  • Jonathan S. Dordick
    • 1
  1. 1.Department of Chemical and Biochemical Engineering and Center for Biocatalysis and BioprocessingUniversity of IowaIowa City

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