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Supported aqueous-phase enzymatic catalysis in organic media

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Abstract

The use of partially hydrated porous silica particles has been studied as a support for cofactor-dependent enzymatic catalysis in organic solvents. At an optimal pore hydration corresponding to 70% pore volume, horse liver alcohol dehydrogenase catalyzes the oxidation and reduction of alcohols and aldehydes, respectively, with rates sixfold higher than with nonporous glass beads as the enzymatic support and with cofactor recycling numbers in excess of 105. Thus, supported aqueous-phase enzymatic catalysis makes highly effective use of the enzyme and cofactor by coimmobilization and by providing a high interfacial area for reactions in organic media.

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Correspondence to Sanghamitra Parida or Ravindra Datta or Jonathan S. Dordick.

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Parida, S., Datta, R. & Dordick, J.S. Supported aqueous-phase enzymatic catalysis in organic media. Appl Biochem Biotechnol 33, 1–14 (1992). https://doi.org/10.1007/BF02922179

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Index Entries

  • Supported liquid-phase catalysis
  • alcohol dehydrogenase
  • organic media