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Supported aqueous-phase enzymatic catalysis in organic media

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Abstract

The use of partially hydrated porous silica particles has been studied as a support for cofactor-dependent enzymatic catalysis in organic solvents. At an optimal pore hydration corresponding to 70% pore volume, horse liver alcohol dehydrogenase catalyzes the oxidation and reduction of alcohols and aldehydes, respectively, with rates sixfold higher than with nonporous glass beads as the enzymatic support and with cofactor recycling numbers in excess of 105. Thus, supported aqueous-phase enzymatic catalysis makes highly effective use of the enzyme and cofactor by coimmobilization and by providing a high interfacial area for reactions in organic media.

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References

  1. Dordick, J. S. (1989),Enz. Microb. Technol. 11, 194–211.

    Article  CAS  Google Scholar 

  2. Klibanov, A. M. (1990),Ace. Chem. Res. 23, 114–120.

    Article  CAS  Google Scholar 

  3. Zaks, A. and Klibanov, A. M. (1988),J. Biol. Chem. 263, 8017–8021.

    CAS  Google Scholar 

  4. Gorman, L. S. and Dordick, J. S. (1992),Biotechnol. Bioeng. 38, 392–397.

    Article  Google Scholar 

  5. Clark, D. S., Skerker, P. S., Creagh, L., Guinn, M., Prausnitz, J., and Blanch, H. W. (1989),ACS Symp. Ser. 392.

  6. Deetz, J.S. and Rozzell, J. D. (1988),Trends Biotechnol. 6, 15.

    Article  CAS  Google Scholar 

  7. Grunwald, J., Wirtz, B., Scollar, M. P., and Klibanov, A. M. (1986),J. Am. Chem. Soc. 108, 6732–6742.

    Article  CAS  Google Scholar 

  8. Wong, C.-H. (1987),Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., pp. 197–208.

  9. Deetz, J. S. and Rozzell, J. D. (1991),Biocatalysts for Industry, Dordick, J. S., ed., pp. 181–191.

  10. Carrea, G. (1984),Trends Biotechnol. 2, 102–106.

    Article  CAS  Google Scholar 

  11. Bückmann, A. F., Morr, M., and Kula, M. R. (1987),Biotechnol. Appl. Biochem. 9, 258–268.

    Google Scholar 

  12. Sekhar, V. C. and Plapp, B. V. (1990),Biochemistry 29, 3289–4295.

    Article  Google Scholar 

  13. Eliel, E. L. and Freeman, J. P. (1952),J. Am. Chem. Soc. 74, 923–927.

    Article  CAS  Google Scholar 

  14. Rony, P. R. (1969),J. Catal. 14, 142.

    Article  CAS  Google Scholar 

  15. Datta, R. and Rinker, R. G. (1985),J. Catal. 95, 181–192.

    Article  CAS  Google Scholar 

  16. Ashamut, J. P., Davis, M. E., Mirola, J. S., and Hanson, B. E. (1989),Nature 339, 454–455.

    Article  Google Scholar 

  17. Laane, C., Boeren, S., Hilhorst, R., and Veeger, C. (1987),Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., pp. 65–84.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering and Center for Biocatalysis and Bioprocessing, University of Iowa, 52242, Iowa City, IA

    Sanghamitra Parida, Ravindra Datta & Jonathan S. Dordick

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  1. Sanghamitra Parida
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  2. Ravindra Datta
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  3. Jonathan S. Dordick
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Parida, S., Datta, R. & Dordick, J.S. Supported aqueous-phase enzymatic catalysis in organic media. Appl Biochem Biotechnol 33, 1–14 (1992). https://doi.org/10.1007/BF02922179

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  • DOI: https://doi.org/10.1007/BF02922179

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