The use of partially hydrated porous silica particles has been studied as a support for cofactor-dependent enzymatic catalysis in organic solvents. At an optimal pore hydration corresponding to 70% pore volume, horse liver alcohol dehydrogenase catalyzes the oxidation and reduction of alcohols and aldehydes, respectively, with rates sixfold higher than with nonporous glass beads as the enzymatic support and with cofactor recycling numbers in excess of 105. Thus, supported aqueous-phase enzymatic catalysis makes highly effective use of the enzyme and cofactor by coimmobilization and by providing a high interfacial area for reactions in organic media.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Dordick, J. S. (1989),Enz. Microb. Technol. 11, 194–211.
Klibanov, A. M. (1990),Ace. Chem. Res. 23, 114–120.
Zaks, A. and Klibanov, A. M. (1988),J. Biol. Chem. 263, 8017–8021.
Gorman, L. S. and Dordick, J. S. (1992),Biotechnol. Bioeng. 38, 392–397.
Clark, D. S., Skerker, P. S., Creagh, L., Guinn, M., Prausnitz, J., and Blanch, H. W. (1989),ACS Symp. Ser. 392.
Deetz, J.S. and Rozzell, J. D. (1988),Trends Biotechnol. 6, 15.
Grunwald, J., Wirtz, B., Scollar, M. P., and Klibanov, A. M. (1986),J. Am. Chem. Soc. 108, 6732–6742.
Wong, C.-H. (1987),Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., pp. 197–208.
Deetz, J. S. and Rozzell, J. D. (1991),Biocatalysts for Industry, Dordick, J. S., ed., pp. 181–191.
Carrea, G. (1984),Trends Biotechnol. 2, 102–106.
Bückmann, A. F., Morr, M., and Kula, M. R. (1987),Biotechnol. Appl. Biochem. 9, 258–268.
Sekhar, V. C. and Plapp, B. V. (1990),Biochemistry 29, 3289–4295.
Eliel, E. L. and Freeman, J. P. (1952),J. Am. Chem. Soc. 74, 923–927.
Rony, P. R. (1969),J. Catal. 14, 142.
Datta, R. and Rinker, R. G. (1985),J. Catal. 95, 181–192.
Ashamut, J. P., Davis, M. E., Mirola, J. S., and Hanson, B. E. (1989),Nature 339, 454–455.
Laane, C., Boeren, S., Hilhorst, R., and Veeger, C. (1987),Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., pp. 65–84.
About this article
Cite this article
Parida, S., Datta, R. & Dordick, J.S. Supported aqueous-phase enzymatic catalysis in organic media. Appl Biochem Biotechnol 33, 1–14 (1992). https://doi.org/10.1007/BF02922179
- Supported liquid-phase catalysis
- alcohol dehydrogenase
- organic media