Abstract
A new strain of the fungusMorchella nov. ES-1 (ATCC 20951) that produces blue pigment in submerged culture has been isolated. The blue pigment was extracted by chloroform or ethyl acetate treatment. The crude extract when chromatographed yielded three fractions; a blue one has been identified by mass spectrometry as indigotin. Fermentation studies using enriched media revealed that maximum accumulation of indigotin in culture was approx 24 mg/L in 168 h.
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References
Gray, P. H. (1972),Roy. Soc. Proc. 102, 263–280.
Sebck, O. and Jaeger, H. (1962),Nature 196, 793–795.
Fujioka, M. and Wada, H. (1968),Biochemica et Biophysica Ada 158, 70–78.
Botsford, J. L. and DeMoss, R. D. (1972),J. Bacterial. 105, 303–312.
Ensley, B. D. (1985), U.S. Patent 4,520,103.
Papazin, H. P. (1950),Botanical Gazette 112, 143–163.
Miles, P. G., Lund, H., and Raper, J. R. (1956),Arch. Biochem. Biophys. 62, 1–5, 1956.
Epstein, E. and Miles, P. G. (1966),Bot. Mag. Tokyo,79, 566–571.
Swack, N. S. and Miles, P. G. (1960),Mycologia. 52, 574–582.
Falangle, H. and Bobbio, P. A. (1962),Arch. Biochem. Biophys. 96, 430–433.
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Eyal, J., Mabud, M.A. & Walter, J.F. Production of indigotin in submerged culture using morchella nov. ES-1. Appl Biochem Biotechnol 30, 303–312 (1991). https://doi.org/10.1007/BF02922034
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DOI: https://doi.org/10.1007/BF02922034