Abstract
Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase fromTrichodertna viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme.
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Abbreviations
- E ads :
-
concentration of adsorbed enzyme (mg-protein/mL)
- Et :
-
total concentration of enzyme (mg-protein/mL)
- k :
-
overall inhibition constant in Eq. (1) (dimensionless)
- m :
-
Parameter in Eq. (2) (dimensionless)
- n :
-
parameter in Eq. (3) (dimensionless)
- n 0 :
-
parameter in Eq. (4) (dimensionless)
- q :
-
parameter in Eq. (4) (dimensionless)
- S o :
-
initial concentration of cellulose (mg/mL)
- t :
-
reaction time (h)
- V :
-
hydrolysis rate of cellulose (mg/mL/h)
- V 0 :
-
initial hydrolysis rate of cellulose (mg/mL/h)
- X :
-
fractional conversion in the hydrolysis of cellulose (dimensionless)
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Ooshima, H., Kurakake, M., Kato, J. et al. Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis. Appl Biochem Biotechnol 31, 253–266 (1991). https://doi.org/10.1007/BF02921752
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DOI: https://doi.org/10.1007/BF02921752