Abstract
Urease has been purified from the seeds ofCajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.
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Pandey, P.C., Pandey, V. Urease purification from the seeds ofCajanus Cajan and its application in a biosensor construction. Appl Biochem Biotechnol 31, 247–251 (1991). https://doi.org/10.1007/BF02921751
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DOI: https://doi.org/10.1007/BF02921751