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Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay

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Abstract

A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease potection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.

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Authors and Affiliations

  1. Developmental Biology Research Centre, King's College, University of London, 26/29 Drury Lane, WC2B 5RL, UK

    Maggie E. Walmsley & Roger K. Patient

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  1. Maggie E. Walmsley
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  2. Roger K. Patient
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Walmsley, M.E., Patient, R.K. Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay. Mol Biotechnol 1, 265–275 (1994). https://doi.org/10.1007/BF02921694

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  • DOI: https://doi.org/10.1007/BF02921694

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