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High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides

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Abstract

High-temperature gas chromatography and gas chromatography-inass spectrometry for the analyses of oligosaccharides derived from glycoproteins or glycosphingolipids has been developed. Pcrmethylatcd oligosaccharides with up to about 12 sugar residues and masses up to 2500 Daltons can be analyzed. This approach is discussed and exemplified.

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Abbreviations

GC:

gas chromatography

MS:

mass spectrometry

GC-MS:

gas chromatography-mass spectrometry

GSL:

glycosphingolipid

Hex:

hexose

HexNAc:

N-acetylhexosamine

HexNAcol:

N-acetylhexosaminitol

Cer:

ceramide

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Authors and Affiliations

  1. Department of Medical Biochemistry, University of Göteborg, Medicinarergatan 9, S-413 90, Göteborg, Sweden

    Hasse Karlsson, Niclas Karlsson & Gunnar C. Hansson

Authors
  1. Hasse Karlsson
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  2. Niclas Karlsson
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  3. Gunnar C. Hansson
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Karlsson, H., Karlsson, N. & Hansson, G.C. High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides. Mol Biotechnol 1, 165–180 (1994). https://doi.org/10.1007/BF02921557

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  • DOI: https://doi.org/10.1007/BF02921557

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