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Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain

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Abstract

An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigen icily of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, 70803, Baton Rouge, LA

    Fan Lin & Gary E. Wise

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  1. Fan Lin
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  2. Gary E. Wise
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Lin, F., Wise, G.E. Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain. Mol Biotechnol 1, 147–150 (1994). https://doi.org/10.1007/BF02921555

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  • DOI: https://doi.org/10.1007/BF02921555

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