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Expression vectors for the construction of hybrid Ty-VLPs

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Abstract

Purification of expressed proteins can be facilitated by expressing the rccombinant protein as a fusion with a carrier protein that assembles into paniculate structures. This article describes the use of expression vectors in producing a hybrid of the yeast retrotransposon Ty, which self-assembles into virus-like particles (VLPs). Hybrid VLPs can be used in such laboratory applications as the production of polyclonal and monoclonal antibodies, structure/function analyses, the detection of important antigenic determinants, and cpitope mapping of monoclonal antibodies.

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Authors and Affiliations

  1. Immunotherapeutics, British Biotechnology Ltd., Wattington Road, 0X4 5LY, Cowley, Oxford, UK

    Sally E. Adams, S. Mark H. Richardson, Susan M. Kingsman & Alan J. Kingsman

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  1. Sally E. Adams
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  2. S. Mark H. Richardson
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  3. Susan M. Kingsman
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  4. Alan J. Kingsman
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Adams, S.E., Richardson, S.M.H., Kingsman, S.M. et al. Expression vectors for the construction of hybrid Ty-VLPs. Mol Biotechnol 1, 125–135 (1994). https://doi.org/10.1007/BF02921553

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  • DOI: https://doi.org/10.1007/BF02921553

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