Conclusions
Membrane filtration is a suitable method for cell harvesting and clarification of fermentation broths. Hollow fiber ultrafilters gave essentially 100% rejection ofL. bulgaricus cells from a whey permeate fermentation broth. A combination of low pressures and high velocity generally gave the best permeate flux. Fermentation media components (in this case, from the whey permeate) contributed significantly to fouling. Considering the pressure limitations of the current generation of asymmetric hollow fiber modules and the changes in physical properties of the fermentation broths, a cell concentration of 100–150 g/L could be obtained with the flux still relatively high (above 20LMH), although the chemical compatibility of the membrane module itself under long-term exposure to high acid conditions should be considered.
Similar content being viewed by others
References
Michaels, A. S. (1980),Desalination 35, 329.
Atkinson, B., and Sainter, P. (1982),J. Chem. Tech. Biotechnol. 32, 100.
Cheryan, M. (1986),Ultrafiltration Handbook, Technomic, Lancaster, PA.
Tutunjian, R. S. (1984),Dev. Ind. Microbiol. 25, 415.
Sims, K. A., and Cheryan, M. (1986),Biotechnol. Bioeng. Symp. 17, 495.
Patel, P. N., Mehaia, M. A., and Cheryan, M. (1987),J. Biotechnol. 5, 1.
Hanisch, W. (1986), inMembrane Separations in Biotechnology, McGregor W. C., ed., Marcell-Decker, New York, pp. 61–88.
Lasky, M., and Grant, D. (1985),Am. Biotechnol. Lab. 3(6), 16.
Gabler, F. R. (1984),Dev. Ind. Microbiol. 25, 381.
Cheryan, M., and Mehaia, M. A. (1985), inMembrane Separations in Biotechnology, McGregor W. C., ed., Marcell-Decker, New York, pp. 255–310.
Khorakiwala, K. H., Cheryan, M., and Mehaia, M. A. (1985),Biotechnol. Bioeng. Symp. 15, 249.
Cheryan, M., and Mehaia, M. A. (1986),CHEMTECH,16, 676.
Kuo, K. P., and Cheryan, M. (1983),J. Food Sci. 48, 1113.
Tejayadi, S. (1987),M. S. thesis, University of Illinois, Urbana, IL.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tejayadi, S., Cheryan, M. Downstream processing of lactic acid-whey permeate fermentation broths by hollow fiber ultrafiltration. Appl Biochem Biotechnol 19, 61–70 (1988). https://doi.org/10.1007/BF02921465
Issue Date:
DOI: https://doi.org/10.1007/BF02921465