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Control of root and shoot formation and production of trees from poplar callus

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Biologia Plantarum


The technique of trees production from the undifferentiated poplar callus tissue is described. The best root formation was observed on the modifiedWolter andSkoog medium when NAA in concentration 0.2 to 0.4 mg l−1 was used as an auxin and cytokinins were omitted. The induction of leafy shoots from the undifferentiated callus was the most effective on the modifiedLinsmaier andSkoog medium in the absence of auxin and with 0.15 to 0.70 mg l−1 of BAP. The best development of roots at the basal end of excised shoots was achieved when shoots were transferred into the sterile mixture of perlit and sand (3: l, v/v) containing a modifiedWolter andSkoog medium.

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  • Chalupa, V. Durzan, D. J.: Growth of Norway spruce (Picea abies (L.)Karst.) tissue and cell cultures.—Commun. Inst. Forest. Čechoslov.8: 109–123, 1973.

    Google Scholar 

  • Jacquiot, C.: Application de la technique de culture des tissus végétaux à l'étude de quelques problèmes de la physiologie de l'arbre.—Ann. Sci. Forest.21: 310–473, 1964.

    Article  Google Scholar 

  • Linsmaier, E. M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures.—Physiol. Plant.18: 100–127, 1965.

    Article  CAS  Google Scholar 

  • Winton, L. L.: Shoot and tree production from aspen tissue cultures.—Amer. J. Bot.58: 904–909, 1970.

    Article  Google Scholar 

  • Winton, L. L.: Tissue culture propagation of European aspen.—For. Sci.17: 348–350, 1971.

    Google Scholar 

  • Wolter, K. E.: Root and shoot initiation in aspen callus cultures.—Nature219: 509–510, 1968.

    Article  CAS  PubMed  Google Scholar 

  • Wolter, K. E., Skoog, F.: Nutritional requirements ofFraxinus callus cultures.—Amer. J. Bot.53: 263–269, 1966.

    Article  Google Scholar 

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Address: 255 01, Zbraslav-Strnady, Czechoslovakia.

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Chalupa, V. Control of root and shoot formation and production of trees from poplar callus. Biol Plant 16, 316–320 (1974).

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