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A strain of Holmes' ribgrass virus occurring in Yugoslavia

Kmen Holmes ribgrass viru vyskytující se v Jugoslávii

  • Published:
Biologia Plantarum

Abstract

A virus having 300 nm long rod-shaped particles was isolated fromPlantago media L. in Yugoslavia. The virus was transmitted to 15 species of host plants the symptoms of which are described in detail. The symptoms corresponded to those that appeared after infection by the original Holmes' ribgrass virus (HRV).

The investigated virus was compared both with the common strain of tobacco mosaic and the original Holmes' ribgrass viruses by means of serological tests. The agar double-diffusion tests showed that it is closely related to HRV and remotely related to the common strain of TMV. On the basis of these results we concluded that this virus represented a strain of the HRV.

The investigations of the cell inclusions showed that our virus produced rounded plates instead of hexagonal prisms. Electron micrographs of ultrathin sectioned material demonstrated that these plates were formed by virus particles lying perpendicularly to the layers of the plates. The presence of plates also points to the fact that the investigated virus belongs to the HRV.

Abstract

Virus tvořený tyčinkovitými částicemi o délce 300 nm byl isolován v Jugoslávii zPlantago media L. Virus byl přenesen na 15 druhů hostitelských rostlin a jejich příznaky jsou podrobně popsány. Symptomy odpovídají těm, které se objevují po infekci originálním Holmes ribgrass virem (HRV).

Serologickými testy byl zkoumaný virus porovnán s běžným kmenem viru tabákové mosaiky as originálním Holmes ribgrass virem. Testy dvojnásobné diffuse v agaru ukázaly, že má těsný vztah k HRV a vzdálený k běžnému kmenu TMV. Na základě těchto výsledků bylo usouzeno, že isolovaný kmen představuje jeden kmenů HRV.

Prozkoumání buněčných inklusí ukázalo, že virus vytváří kruhovité terčíky místo hexagonálních hranolů. Elektronmikroskopické studie ultratenkých řezů osvětlily, že tyto terčíky jsou tvořeny virovými částicemi ležícími kolmo na rovinu terčíků. Přítomnost terčíků rovněž potvrzuje, že zkoumaný kmen náleží k HRV.

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Authors and Affiliations

  1. Botanical Institute of Zagreb University, Zagreb

    N. Juretić

  2. Institute “Rudjer Bošković”, Zagreb

    Mercedes Wrischer

  3. Department of Plant Pathology, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    Z. Polák

Authors
  1. N. Juretić
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  2. Mercedes Wrischer
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  3. Z. Polák
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Additional information

Address: Marulićev trg 20/II, Zagreb, Yugoslavia.

Address: Bijenička cesta 54, Zagreb, Yugoslavia.

Address: Na Karlovce 1, Praha 6, Czechoslovakia.

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Juretić, N., Wrischer, M. & Polák, Z. A strain of Holmes' ribgrass virus occurring in Yugoslavia. Biol Plant 11, 284–290 (1969). https://doi.org/10.1007/BF02921145

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  • DOI: https://doi.org/10.1007/BF02921145

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