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Peroxidase activities and contents of phenolic acids in embryogenic and nonembryogenic alfalfa cell suspension cultures

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Biologia Plantarum

Abstract

Contents of phenolic acids, peroxidase activities and growth curves showed significant differences in embryogenic (EC) and nonembryogenic (NEC) suspension cultures ofMedicago sativa L. NEC gave a typical growth curve while in EC the distinct phases were absent. The total content of phenolic acids was higher in NEC (related to EC), changed during the growth cycle and most of the acids occurred in ester-bound methanol soluble form. The level of phenolic acids in EC was significantly lower and did not change during 12-d cultivation. The major fraction was formed by phenolic acids ester-bound to the cell wall. The cytoplasmic peroxidase activity in NEC increased continuously during the growth and reached the maximum value at the end of exponential phase. In EC the extremely low cytoplasmic peroxidase activity did not change during cultivation. Ionically bound peroxidases in NEC represented 14 to 30% of the total extracted activity in dependence on the growth phase while in EC formed about 50% of the total activity and did not change during studied period. A possible participation of ionic peroxidase in the incorporation of phenolics into the cell wall is discussed.

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Abbreviations

EC:

embryogenic suspension culture

NEC:

nonembryogenic suspension culture

BL:

medium of Blaydes

2,4-D:

2,4-dichlorophenoxyacetic acid

PO:

peroxidase activity

FM:

fresh mass

References

  • Binarová, P., Doležel, J.: Alfalfa embryogenic cell suspension culture: Growth and ploidy level stability.—J. Plant Physiol.133: 561–566, 1988

    Google Scholar 

  • Blaydes, D.F.: Interaction of kinetin and various inhibitors of the growth of soybean tissue.—Physiol. Plant.19: 748–753, 1966.

    Article  CAS  Google Scholar 

  • Bouazza, A., Rambour, S., Gaspar, T., Legrand, B.: Peroxidases during the course of callusing and organ differentiation from root explants ofCichorium intybus.—Biol. Plant.35: 481–489, 1993.

    Article  CAS  Google Scholar 

  • Cordewener, J., Booij, H., Van der Zandt, H., Van Engelen, F., Van Kammen, A., De Vries, S.: Tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes.—Planta184: 478–486, 1991.

    Article  CAS  Google Scholar 

  • Cvikrová, M., Hrubcová, M., Meravý, L., Pospíšil, F.: Changes in the content of phenolic substances during the growth ofNicotiana tabacum cell suspension culture.—Biol. Plant.30: 185–192, 1988.

    Article  Google Scholar 

  • Cvikrová, M., Meravý, L., Machácčková, I., Eder, J.: Phenylalanine ammonia-lyase, phenolic acids and ethylene in alfalfa (Medicago sativa L.) cell cultures in relation to their embryogenic ability.— Plant Cell Rep.10: 251–255, 1991.

    Article  Google Scholar 

  • Ferrer, M.A., Pedreño, M.A., Muñoz, R., Ros Barceló, A.: Soluble peroxidase gradients in lupin hypocotyls and the control of the level of polarly transported indole-3yl-acetic acid—J. Plant Growth Regul.10: 139–146, 1991.

    Article  CAS  Google Scholar 

  • Gaspar, T., Penel, C., Castillo, F.J., Greppin, H.: A two-step control of basic and acidic peroxidases and its significance for growth and development.—Physiol. Plant.64: 418–423, 1985.

    Article  CAS  Google Scholar 

  • Grambow, H.J., Langebeck-Schwich, B.: The relationship between oxidase activity, peroxidase activity, hydrogen peroxide, and phenolic compounds in the degradation of indole-3-acetic acidin vitro.—Planta157: 131–137, 1983.

    Article  CAS  Google Scholar 

  • Pedreño, M.A., Ros-Barceló, A., Garcia-Carmona, F., Muñoz, R.: Oxidation of dihydroxyfumaric acid in the absence of H2O2 by cell wall-bound peroxidases from lupin: A possible general model.— Plant Physiol. Biochem.28: 37–42, 1990.

    Google Scholar 

  • Ros Barceló, A., Muñoz, R., Sabater, F.: Substrate specifity of soluble lupin isoperoxidases.— Biol. Plant.31: 161–167, 1989.

    Google Scholar 

  • Van Engelen, F.A., De Vries, S.C.: Extracellular proteins in plant embryogenesis.—Trends Genet.8: 66–70, 1992.

    PubMed  Google Scholar 

  • Zheng, X., Van Huystee, R.B.: Peroxidase-regulated elongation of segments from peanut hypocotyls. —Plant Sci.81: 47–56, 1992.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Ke dvoru 15, 166 30 Praha 6, Czech Republic

    M. Hrubcová, M. Cvikrová & J. Eder

Authors
  1. M. Hrubcová
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  2. M. Cvikrová
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  3. J. Eder
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Communicated by J. TYPÝ

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Hrubcová, M., Cvikrová, M. & Eder, J. Peroxidase activities and contents of phenolic acids in embryogenic and nonembryogenic alfalfa cell suspension cultures. Biol Plant 36, 175–182 (1994). https://doi.org/10.1007/BF02921083

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  • DOI: https://doi.org/10.1007/BF02921083

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