Skip to main content
SpringerLink
Log in

Histochemical investigation of dehydrogenases in shoot apices of wheat plants at different ontogenetic stages

Histochemické studium dehydrogenas ve vzrostných vrcholech pšenice

  • Published:
Biologia Plantarum

Abstract

The activity and localization of alcohol (AD), lactic (LD), malic (MD), isocitric (ICD) and succinic (SD) dehydrogenase, resp. was histochemically determined in shoot apices of plants in the vegetative condition during transition to flowering and at the reproductive state. The enzymes were determined in freehand sections, as well as in shoot apices incubatedin toto according toNachlas et al. (1958) using Nitro-BT. The characteristic localization of the enzymes depending on the ontogenic stage of the shoot apex is described. Different parts, layers and cell groups of the apex showed differences in enzyme activity. SD could not be detected in shoot apices. The histochemical findings are discussed in relation to former results obtained from a biochemical investigation of respiration in the same material.

Abstract

Ve vzrostných vrcholech rostlin vegetativních, přecházejících ke kvetení a generativních byla histochemicky sledována aktivita a lokalisace dehydrogenasy alkoholu (AD), kyseliny mléčné (LD), kyseliny jablečné (MD), kyseliny isocitronové (ICD) a kyseliny jantarové (SD), (v ručních řezech a vzrostných vrcholech inkubovaných in toto metodou podleNachlase a spol. 1958 s Nitro-BT). Byla popsána charakteristická lokalisace enzymů související s určitou ontogenetickou etapou vzrostného vreholu: vysoká aktivita LD u juvenilních vzrostných vrcholů, vysoká aktivita AD, ICD a LD ve skupině terminálních iniciál vegetativních vrcholů, změna v lokalisaci AD v základech klásků generativních vrcholů oproti lokalisaci v mladších pupenech. Z hlediska diferenciace meristematických buněk pletiva vzrostného vrcholu byly zjištěny některé rozdíly v aktivitě sledovaných enzymů v různých částech, vrstvách, resp. skupinách buněk. SD nebyla ve vzrostných vrcholech prokázána vůbec. Histochemické nálezy jsou diskutovány v souvislosti s dřívějšími výsledky biochemického studia respirace téhož materiálu.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Avers, Ch. J., Tkal, M.: Intracellular mitochondrial variation in enzyme activity as shown by histochemical studies using light and electron microscopy.—J. Histochem. Cytochem.11: 157–162, 1963.

    Article  CAS  Google Scholar 

  • Barnard, C.: Histogenesis of the inflorescence and flower ofTriticum aestivum.—Aust. J. Bot.3: 1–20, 1955.

    Article  Google Scholar 

  • Beneš, K., Lojda, Z., Hořavka, B.: A contribution to the histochemical demonstration o some hydrolytic and oxidative enzymes in plants.—Histochemie2: 313–321, 1961.

    Article  PubMed  Google Scholar 

  • Ekés, M.: Electron-microscopic-histochemical demonstration of succinic-dehydrogenase activity in root cells of yellow Lupine.—Planta94: 37–46, 1970.

    Article  PubMed  Google Scholar 

  • Esau, K.: Plant anatomy.—Wiley, New York 1953.

    Google Scholar 

  • Evans, L. S., Berg, A. R.: Early histogenesis and semiquantitative histochemistry of leaf initiation inTriticum aestivum.—Amer. J. Bot.59: 973–981, 1972.

    Article  Google Scholar 

  • Fosket, D. E., Miksche, J. P.: A histochemical study of the seedling shoot apical meristem ofPinus lambertiana.—Amer. J. Bot.53: 694–702, 1966.

    Article  CAS  Google Scholar 

  • Knox, R. B., Evans, L. T.: Inflorescence initiation inLolium temulentum L. VII. Histochemical changes at the shoot apex during induction.—Aust. J. Biol. Sci.19: 223–245, 1966.

    Google Scholar 

  • Liu, H. S., Baker, B. L.: The influence of technique and hormones on histochemical demonstration of enzyme in intestinal epithelium.—J. Histochem. Cytochem.11 (3): 349–364, 1963.

    Article  CAS  Google Scholar 

  • Lyndon, R. F.: Growth of the surface and inner parts of the pea shoot apical meristem during leaf initiation.—Ann. Bot.35: 263–270, 1971.

    Google Scholar 

  • Mia, A. J., Pathlak, S. M.: A histochemical study of the shoot apical meristem of Rauwolfia with reference to differentiation of sclereids.—Can. J. Bot.46: 115–120, 1968.

    Article  Google Scholar 

  • Nachlas, M. M., Walker, D. G., Seligman, A. M.: A histochemical method for demonstration of diphosphopyridine nucleotide diaphorase.—J. Biophys. Biochem. Cytol.4 (1): 29–37, 1958.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Opatrná, J.: Cytological and cytochemical enzyme studies of the shoot apex of wheat.—In: Proc. Symp. Differentiation of Apical Meristems. Praha-Nitra 1964. Pp. 129–130. Academia, Praha 1966.

    Google Scholar 

  • Opatrná, J.: Histochemical localization of some dehydrogenases in the shoot meristem of wheat at different stages of development.—In:Bernier, G. (ed.): Cellular and Molecular Aspects of Floral Induction. Pp. 80–90, Longman Publ., London, 1970.

    Google Scholar 

  • Opatrná, J.: Selective histochemical localisation of alcohol dehydrogenase in bud primordia cells of wheat shoot apices.—Biol. Plant.16: 149–151, 1974.

    Article  Google Scholar 

  • Opatrná, J., Seidlová, F., Beneš, K.: The anatomy of the shoot apex of wheat (Triticum aestivum L.) during transition from the vegetative to the reproductive state and the determination of the primordia.—Biol. Plant.6: 219–225, 1964.

    Article  Google Scholar 

  • Owens, J. N.: The relative importance of initiation and early development on cone production of Douglas fir.—Can. J. Bot.47: 1039–1049, 1969.

    Article  Google Scholar 

  • Poux, N.: Comportement du méristème apical d'un Blé d'hiver (Triticum sativum Lamk.) semé au printemps.—Compt. rend. Acad. Sci. (Paris) D245: 2522–2525, 1957.

    Google Scholar 

  • Poux, N.: Variation de la répartition des ribonucléoprotéines dans les apex d'un Blé d'hiver (Triticum sativum Lamb.) semé soit au printemps, soit en automne.—Compt. rend. Acad. Sci. (Paris) D251: 1904–1906, 1960.

    CAS  Google Scholar 

  • Riding, R. T., Gifford, E. M.: Histochemical changes occuring at the seedling shoot apex ofPinus radiata.—Can. J. Bot.51 (1): 501–513, 1973.

    Article  CAS  Google Scholar 

  • Teltscherová, L.: Über die Veränderungen einiger Stoffwechselprodukte in den Vegetationskegeln von Getreide im Laufe der Entwicklung der Pflanzen.—Rozpravy ČSAV (Praha)12: 1–146, 1962.

    Google Scholar 

  • Teltscherová, L., Dvořák, M.: Über die anaeroben Stoffwechsel der Vegetationskegel des Weizens.—Biol. Plant.5: 19–28, 1963.

    Article  Google Scholar 

  • Teltscherová, L., Krekule, J.: Zum Einfluss einiger Inhibitoren der Glykolyse und der Atmung auf den Gehalt an Zuckern und Gärungsprodukten in Vegetationskegeln von Weizenpflanzen auf verschiedenen Entwicklungsstufen der Pflanzen.—Biol. Plant.6: 42–47, 1964a.

    Article  Google Scholar 

  • Teltscherová, L., Krekule, J.: Beitrag zum Studium des Citronensäurencyklus in Vegetationskegeln von Weizenpflanzen von verschiedenem Entwicklungsalter.—Biol. Plant.6: 279–284, 1964b.

    Article  Google Scholar 

  • Teltscherová, L., Krekule, J.: Bestimmung des Respirationsquotienten an Vegetationskegeln von Weizenpflanzen auf verschiedenen Entwicklungsstufen der Pflanzen.—Biol. Plant.9: 75–78, 1967.

    Article  Google Scholar 

  • Thielke, Ch.: Strukturwechsel und Enzymmuster am Sproßscheitel einiger Gräser.—Planta66: 310–319, 1965.

    Article  CAS  Google Scholar 

  • Tiefel, R. M.: Histochemical Differentiation of Meristems.—Thesis, University of Missouri 1957.

  • Vanden Born, W. H.: Histochemical studies of enzyme distribution in shoot tips of white spruce (Picea glauca (Moench) Voss).—Can. J. Bot.43: 1509–1527, 1963.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Growth and Development, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    Jana Opatrná

Authors
  1. Jana Opatrná
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Address: Ke dvoru 16, 160 30 Praha 6-Vokovice, Czechoslovakia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Opatrná, J. Histochemical investigation of dehydrogenases in shoot apices of wheat plants at different ontogenetic stages. Biol Plant 17, 67–74 (1975). https://doi.org/10.1007/BF02921077

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02921077

Additional index words

Search

Navigation