Abstract
We studied the significance of actual accessibility of ions for presence of α-amylase in roots and cotyledons of young pumpkin plants. We considered α-amylases to be—in agreement with published data—amylolytic enzymes which lose their activity after being dialysed against EDTA or a dilute solution of hydrochloric acid and which can be reactivated by addition of Ca2+. N connection with activity and Ca2+ nutrition appeared in cotyledons; it did, however, in roots: Ca-deficient plants revealed after preparation either completely inactive enzymes or enzymes with slight activity. It was, however, possible to reactivate them by addition of Ca2+ during preparation.
So, using polyacrylamide disc electrophoresis we present evidence for the appearence of amylase with typical characteristics for α-amylases of other sources. Its relative mobility was the same for isolates from both roots and cotyledons. Its biosynthesis was not dependent upon metal which is associated with enzyme. No conclusion can be drawn as to whether Ca-deficient α-amylases are activein vivo; therefore it was impossible to judge whether α-amylase activity is subject to feed-back regulation.
Abstract
V souvislosti se studiem funkce Ca2+ v metabolismu rostliny jsme sledovali význam aktuální dostupnosti iontu pro výskyt α-amyláz v kořenech a dělohách mladých rostlin tykve. Za α-amylázy jsme považovali—shodně s údaji v literatuře—amylolytické enzymy, které ztrácejí aktivitu dialýzou proti EDTA či ve zředěné HCl a které je možno reaktivovat adicí Ca2+. V dělohách nebyl pozorovatelný vztah aktivity k výživě vápníkem, v kořenech ano: „*#x2014;Ca” rostliny dávaly po preparaci neaktivní nebo málo aktivní enzym; bylo jej však možno aktivovat přidáním Ca2+ během preparace. Tak jsme prokázali pomocí polyakrylamidové diskové elektroforézy výskyt amylázy s typickými charakteristikami pro α-amylázy jiných objektů. Měla stejnou relativní pohyblivost u kořenů i u děloh. Dále, že její biosyntéza není závislá na kovu enzymu. Práce nemohla řešit otázku, zda jsou Ca-deficientní α-amylázy in vivo aktivní a nelze tedy soudit, zda existuje „feed-back” regulace produktem hydrolytické aktivity.
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Dvořák, M., Radotínská-Ledinská, V. The amylolytic activity of Ca-deficient pumpkin plants (Cucurbita pepo L.). Biol Plant 12, 117–124 (1970). https://doi.org/10.1007/BF02920859
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DOI: https://doi.org/10.1007/BF02920859