Skip to main content
SpringerLink
Log in

Isolation of the protoplasts fromAsteraceae by means of xylonase

  • Brif Communication
  • Published:
Biologia Plantarum

Abstract

The enzyme xylonase was used to isolate the protoplasts from the leaves ofCalendula officinalis L.,Gazania splendens Moore,Tithonia rotundifolia Blake,Zinnia elegans Jacq- and from the petals ofDahlia variabilis (Willd.) Desf. The recovery of spherical undamaged protoplasts differed. The same method did not lead to the isolation of the protoplasts from callus cultures derived fromCalendula, Gazania andTithonia leaves respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Gleba, Yu. Yu., Sytnik, K. M.: [Isolated protoplasts of higher plants.] In Russ.—Dopov. Akad. Nauk URSR, B1973 (10): 942–945, 1973.

    Google Scholar 

  • Landa, Z.: [Long term callus cultures ofDahlia variabilis (Willd.) Desf. andDianthus caryophyllus L.] In Czech.—In:Landa, Z., Novák, F. J., Opatrný, Z. (ed.): 1st Colloquium „Plant Tissue Cultures in Genetics and Breeding”. Pp. 310–318. Praha-Olomoue 1973.

  • Landová, B., Landa, Z.: Organogenesis in callus culture ofGazania splendens Moore induced on new medium.—Experientia30: 832–834, 1974.

    Article  Google Scholar 

  • Potrykus, I.: Intra- and interspecific fusion of protoplasts from petals ofTorenia baillonii andTorenia forenia.—Nature New Biol.231: 57–58, 1971.

    Article  CAS  PubMed  Google Scholar 

  • Wakasa, K.: Isolation of protoplasts from various plant organs.—Japan. J. Genet.48: 279 to 289, 1973.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Theoretical Bases of Breeding Methods, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Czechoslovakia

    Běla Landová & Z. Landa

Authors
  1. Běla Landová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. Landa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Address: Alšovice 69, 468 21 Bratříkov, Jablonee n.N., Czechoslovakia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Landová, B., Landa, Z. Isolation of the protoplasts fromAsteraceae by means of xylonase. Biol Plant 17, 219–222 (1975). https://doi.org/10.1007/BF02920839

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02920839

Keywords

Search

Navigation