Skip to main content
SpringerLink
Log in

Mycoplasma-like bodies in plants infected with potato witches' broom disease and the response of plants to tetracycline treatment

Částice připomínající zástupce roduMycoplasma v rostlinách nakažených metlovitostí bramboru a reakce rostlin na ošetření tetracyklinem

  • Published:
Biologia Plantarum

Abstract

The virus origin of a Czechoslovak isolate of potato witches' broom disease is discounted: electron micrographs of ultrathin section of phloem tissues from plants infected with potato witches' broom disease demonstratedMycoplasma-like bodies, spherical or elongated showing binary fission and fragmentation. The minute corpuscles have a diameter of about 50–60 nm, the largest bodies of 1000 nm. The width of elongated filamentous structures is about 200 nm, most oval bodies have a diameter of 250 nm. A weak tetracycline treatment of diseased plants causes a delay of symptom development; a strong dose of tetracycline (applied by means of the wick method into the stem) inhibits symptom appearance completely. Tetracycline produces a phytotoxic effect inhibiting the growth of tomato plants and causing (at higher concentrations) necrosis and death of these plants. There is a note in the paper dealing with the term “mycoplasma”. The word mycoplasma in the sense ofEriksson (1897) or ofMereschkowsky (1910) does not correspond to the genus nameMycoplasma Nowak (1929).

Abstract

Práce popírá virovou etiologii metlovitosti bramboru: na elektronogramech ultratenkých žezů lýkem rostlin nakažených metlovitostí bramboru byly zjištěny částice připomínajícíMycoplasma, kulovité nebo vláknité, které se větví nebo se z nich odškrcují menší tělíska. Nejmenší tělíska mají průměr 50–60 nm, největší 1000 nm. Vlákna jsou tlustá obvykle kolem 200 nm, oválná tělíska mají nejčastější průměr 250 nm. Slabá dávka tetracyklinu zpožduje vývoj příznaků infikovaných rostlin, silná dávka (po aplikaci tetracyklinu knotem do stonku) úplně zabrání vývoji příznaků metlovitosti. Tetracyklin však působí toxicky na rostliny, inhibuje jejich růst a ve vyšší dávce působí nekrózy i uhynutí. V práci je terminologická poznámka: termín mycoplasma ve smysluErikssonově (1897) ani ve smysluMereschkowského (1910) nesouvisí s rodovým označením mikroorganismůMycoplasma Nowak (1929).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bojňanský, V., Blattný, C., Laffers, E.: K výskytu metlovitosti bramborů v ČSR. [On the occurence of potato witches' broom disease in Czechoslovakia.]— Preslia30: 360, 1958.

    Google Scholar 

  • Brčák, J., Králík, O.:Mycoplasma-like microorganisms and virus particles in the leafhopperJavesella pellucida (F.) transmitting the oat sterile dwarf disease.—Biol. Plant.11: 95–96, 1969.

    Google Scholar 

  • Cousin, M. T., Maillet, P. L., Gourret, J. P., Grison, C., Staron, T.: Présence de particules de type “Mycoplasme” dans le phloème de plantes infectées par trois maladies de type “Jaunisse européenne”: de stolbur, de la tomate, l'aster yellow du glaieul, la phyllodie du trèfle. Études cytologique et ultrastructurale. Premiers essais de lutte chimique.—Acad. Agric. France, Extrait du procès-verbal de la Séance du 9 Octobre pp. 887–895, 1968.

  • Davis, R. E., Whitcomb, R. F., Steere, R. L.: Chemotherapy of aster yellows disease.— Phytopathology58: 884, 1968.

    Google Scholar 

  • Doi, Y., Teranaka, M., Yora, K., Asuyama, H.: Mycoplasma- or PLT group-like micro-organisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows, or Paulownia witches' broom.—Ann. Phytopath. Soc. Japan33: 259–266, 1967.

    Google Scholar 

  • Edward, D. G. F. F., Frundt, E. A.: The classification and nomenclature of organisms of the pleuropneumonia group.—J. gen. Microbiol.14: 197–207, 1956.

    PubMed  CAS  Google Scholar 

  • Eriksson, J.: Vie latente et plasmatique de certaines urédinées.—C. R. Acad. Sci. Paris124: 475, 1897.

    Google Scholar 

  • Eriksson, J.: Über die Mycoplasmatheorie, ihre Geschichte und ihren Tagesstand.—Biol. Cbl.30: 618–623, 1910.

    Google Scholar 

  • Fukushi, T., Shikata, E., Shioda, H., Sekiyama, E., Tanaka L., Oshima, N., Nishia, Y.: Insect transmission of potato witches' broom in Japan.—Proc. Jap. Acad.31: 234–236. 1955.

    Google Scholar 

  • Granados, R. R., Maramorosch, K., Shikata, E.: Mycoplasma: suspected etiologic agent of corn stunt.—Proc. nat. Acad. Sci. (Wash.)60: 841–844, 1968.

    Article  Google Scholar 

  • Ishiie, T., Doi, Y., Yoda, K., Asuyama, H.: Suppressive effects of antibiotics of tetracycline group on symttom development of mulberry dwarf disease.—Ann. Phytopath. Soc. Japan33: 267–275, 1967.

    CAS  Google Scholar 

  • Limberk, J.: Beitrag zur Kenntnis der Stammzusammensetzung der Kartoffelhexenbesen-krankheit.—Preslia32: 407, 1960.

    Google Scholar 

  • Macht, D. I., Livingston, M. B.: Effect of cocaine on the growth ofLupinus albus. A contribution to the comparative pharmacology of animal and plant protoplasm.—J. gen. Physiol.4: 573–584, 1922.

    Article  CAS  Google Scholar 

  • Macovschi, E.: Metoda capilará pentru introducerea compusilor chimici si produsilor biologici în corpul plantelor. [Méthode capillaire pour l'introduction dés composés chimiques et produits biologiques dans le corps des plantes.]—Comunicárile Acad. RP Romîne8: 183–187, 1958.

    CAS  Google Scholar 

  • Maillet, P. L., Gourret, J. P., Hamon, C.: Sur la présence de particules de type Mycoplasme dans le liber de plantes atteintes de maladies du type “jaunisse” (Aster Yellow, Phyllodie du Trèfle, Stolbur de la Tomate) et sur la parenté ultrastructurale de ces particules avec celles trouvées chez divers Insectes Homoptères.—C. R. Acad. Sci. Paris266 (D): 2309–2311, 1968.

    Google Scholar 

  • Maramorosch, K., Shikata, E., Granados, R. R.: Structures resemblingMycoplasma in diseased plants and in insect vectors.—Transactions N. Y. Acad. Sci.30: 841–855, 1968.

    Google Scholar 

  • Mereschkowsky, C.: Theorie der zwei Plasmaarten als Grundlage der Symbiogenesis, einer neuen Lehre von der Entstehung der Organismen.—Biol. Cbl.30: 278–288, 1910.

    Google Scholar 

  • Milne, R.: Electron microscopy of leaves infected with sowbane mosaic virus and other small polyhedral viruses.—Virology32: 589–600, 1967.

    Article  CAS  PubMed  Google Scholar 

  • Nowak, J.: Morphologie, nature et cycle évolutif du microbe de la péripneumonie des bovidés.— Ann. Inst. Pasteur43: 1330–1352, 1929.

    Google Scholar 

  • Raynolds, E. S.: Use of lead citrate at high pH as an electron-opaque stain in electronmicroscopy.— J. Cell Biol.17: 209, 1963.

    Google Scholar 

  • Steinbrecht, R. A., Ernst, K.-D.: Continuous penetration of delicate tissue specimens with embedding resin (24).—Science Tools14(2), August, 1967.

  • Tischler, G.: Kurzer Bericht über die von Eriksson und mir ausgeführten Untersuchungen über das vegetative Leben des Gelbrostes (Puccinia glumarum Erikss. etHenn.).—Biol. Cbl.24: 417–423, 1904.

    Google Scholar 

  • Valenta, V.: Vírus metlovitosti zemiakov v Čeksoslovensku. (Príspevok k otázke “severného” stolburu.) [Potato witches', broom disease in Czechoslovakia.].—Biológia11: 449–456, 1956.

    Google Scholar 

  • Valenta, V.: Otazka “severného stolburu” v Československu. [The problem of “North stolbur” in Czechoslovakia.] Stolbur a príbuzné vírusové bezsemennosti rastlín.—Sb. ved. konf. o stolbure a príbuzných bezsemennostiach, Smolenice1956: 168–177, 1958a.

    Google Scholar 

  • Valenta, V.: Potato witches' broom virus in Czechoslovakia.—Proc. 3rd Conf. Potato Virus Dis., Lisse-Wageningen1957: 246–250, 1958b.

    Google Scholar 

  • Wright, N. S.: Studies on the witches'-broom virus disease of potatoes in British Columbia.—Canad. J. Bot.30: 735–742, 1952.

    Article  Google Scholar 

  • Wright, N. S.: Potato witches' broom in North America.—Proc. 3rd Conf Potato Virus Dis., Lisse-Wageningen1957: 239–245, 1958.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Institute of Experimental Botany, Czechslovak Academy of Sciences, Praha

    J. Brčák, O. Králík, J. Limberk & Marie Ulrychová

Authors
  1. J. Brčák
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. Králík
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Limberk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marie Ulrychová
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Address: Na Karlovce 1, Praha 6, Czechoslovakia.

The plates will be found at the end of the issue.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brčák, J., Králík, O., Limberk, J. et al. Mycoplasma-like bodies in plants infected with potato witches' broom disease and the response of plants to tetracycline treatment. Biol Plant 11, 470–476 (1969). https://doi.org/10.1007/BF02920712

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02920712

Keywords

Search

Navigation