Abstract
Grains ofTriticum monococcum L. var.sofianum Körn. were treated with O.1 mM, 0·2 mM and 0·3 mM solutions of N-nitroso-N-methylurea (MNtt), with 0.03 M solution of buthylmethane sulphonate (BMS) and with X-rays in doses of 5 000r and 10 000r. The germination and development of individual colors of chlorophyl mutants were observed by the system developed byLamprecht (1960). All the mutants induced were classified according to their color changes into three main categories-homogenous unicolor, homogenous multieolor and heterogenous multieolor. In the last type the colors of individual leaves of the same plant varied. Anthocyanin mutations “albina” and “albino-transvirgata” sometimes coincided with the chlorophyll mutations. Some chlorophyll mutations showing complicated groups of colors appeared which were beyond the scale of classification by ordinary systems. The largest proportion in the spectrum of chlorophyll mutations, induced by MNH and X-rays was occupied by mutations of thealbina type. The broadest mutation spectrum in our experiments was induced by the application of 0.3 mM MNH. The doses of X-rays used induced relatively higher numbers of albina-type chlorophyll mutations than MNH and BMS. In our experiments we succeeded in inducing on medium size samples ofTriticum monococcum L. var.sofianum Körn not only almost all types of chlorophyll mutations, induced byFuji (1960, 1962) andMatsumura (1960), but in addition also a great number of other even more complicated chlorophyll mutations, which have never been previously described inTriticum monococcum. L.
Abstract
ObilkyTriticum monococcum L. var.sofianum Körn. byly ovlivněny 0,1 raM, 0,2 mM a 0,3 mM roztokem N-nitroso-N-methylmočoviny (MNH), 0,03 M roztokem buthylmethansulfonátu (BMS) a 5 000 r i 10 000 r paprskd. X V M2 bylo sledovámo vzcházeni a byl zaznamenáván individuální barevný vývoj všech indukovaných chlorofylových mutantú klasifikačním systémem podleLamprechth (1960). Indukovaní mutanti tvořili podle typú barevných změn 3 skupiny – rovnoměrně jednobarevně, rovnoměrně vicebarevné a nerovnoměrně vicebarevné, kde se odlišovaly zbarvením od sebe i jednotlivé listy téhož, individua. U některých chlorofylových mutantú typú albina se objevily anthocyanové mutace typu „albina” a typu „albino transvirgata”. Několik barevně složitěší cblorofylových mutantú nebylo možné podle dosavadnich systémtú klasifikovat. Největší procentuální zastoupení ve spektrech chlorofylových mutací indukovaných MNH a paprsky X připadalo n a mutacialbina. Nejširší mutační spektrum ze všech variant indukovala 0,3 mM koncentrace MNH. Použité dávky paprskú X indukovaly relativně větší počet chlorofylových mutaci albina než MNH a BMS. V práci se podařilo uT. monococcum L. var.sofianum Körn. indukovat na nepřliš velikém materiálu nejen téměřvsechny typy chlorofylových mutací popsané Fujiim (Fuji 1960, 1962) a Matsumurou (Matsumu, 1960) uT. monococcum L. var.flavescens Körn., ale i řadu dalších, zejména složitějších chlorofylových mutaci, uT. monococcum L. doposud nepublikovaných.
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Vagera, J. The effect of N-nitroso-N-methylurea, buthylmethane sulphonate and x-rays on the germination and production of chlorophyll mutations in einkorn wheat. Biol Plant 11, 408–416 (1969). https://doi.org/10.1007/BF02920704
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DOI: https://doi.org/10.1007/BF02920704