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Optimal nutrient feed policies for heterologous protein production

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Abstract

RecombinantEscherichia coli, which overproduce heterologous protein, redirect endogenous metabolic activity to that mediated by the recombinant expression vector. Consequently, cells may experience perturbations in the biosynthetic reaction network, including the amino acid biosynthesis pathways. These cells are characterized by decreased growth rate, decreased cell mass yield, and increased heterologous protein degradation. This study investigates the dynamics of chloramphenicol-acetyl-transferase (CAT) synthesis and degradation inE. coli JM105 grown on minimal media, and correlates the observed phenomena with induction strength. Coordinated amino acid feeding was shown to increase the heterologous protein yield. Rational design of nutrient feeding possibilities is explored.

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Authors and Affiliations

  1. Center for Agricultural Biotechnology, Department of Chemical Engineering, University of Maryland, 20742, College Park, MD

    S. W. Harcum, D. M. Ramirez & W. E. Bentley

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  1. S. W. Harcum
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  2. D. M. Ramirez
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  3. W. E. Bentley
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Harcum, S.W., Ramirez, D.M. & Bentley, W.E. Optimal nutrient feed policies for heterologous protein production. Appl Biochem Biotechnol 34, 161–173 (1992). https://doi.org/10.1007/BF02920543

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  • DOI: https://doi.org/10.1007/BF02920543

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