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Enhancement of 1,3-Propanediol production by cofermentation inEscherichia coli expressingKlebsiella pneumoniae dha regulon genes

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1,3-Propanediol (1,3-PD) is an intermediate in chemical and polymer synthesis. We have previously expressed the genes of a biochemical pathway responsible for 1,3-PD production, thedha regulon ofKlebsiella pneumoniae, inEscherichia coli. An analysis of the maximum theoretical yield of 1,3-PD from glycerol indicates that the yield can be improved by the cofermentation of sugars, provided that kinetic constraints are overcome. The yield of 1,3-PD from glycerol was improved from 0.46 mol/mol with glycerol alone to 0.63 mol/mol with glucose cofermentation and 0.55 mol/mol with xylose cofermentation. The engineeredE. coli also provides a model system for the study of metabolic pathway engineering.

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Tong, IT., Cameron, D.C. Enhancement of 1,3-Propanediol production by cofermentation inEscherichia coli expressingKlebsiella pneumoniae dha regulon genes. Appl Biochem Biotechnol 34, 149–159 (1992).

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