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Depolymerization and chemical modification of lignite coal byPseudomonas cepacia strain DLC-07

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Coal metabolizingPseudomonas cepacia DLC-07 was isolated from soil and shown to use soluble lignite coal as a carbon/energy source.Pseudomonas modified coal residues were characterized by FT-IR, HPLC, elemental analysis, and13C-NMR. P.cepacia DLC-07 depolymerized lignite, as shown by HPLC. FT-IR analysis showed fewer carbonyl and carboxyl groups, decreased etheric oxygen, and fewer aromatic and conjugated carbon-carbon double bonds in modified, compared to control coal.13C-NMR indicated a decrease in carbonyls and heteroaromatic carbons containing N, O, and S, a decrease in unsubstituted aromatic carbons, and an increase in long chain methylenes in treated coal. Elemental analysis supported the spectral data concerning metabolism of the coal polymer. P.cepacia cometabolized coal model compounds and utilizedp-hydroxy substituted benzoic or cinnamic acids or aldehydes as sole carbon and energy sources.

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References

  1. Buchanon, R. E. and Gibbon, N. E. (1974),Bergey’s Manual of Determinative Bacteriology, Williams and Wilkins, New York, p. 232.

    Google Scholar 

  2. Chatterjee, D. K., Kilbane, J. J., and Chakrabarty, A. M. (1982),Appl. Environ. Microbiol. 44, 514.

    CAS  Google Scholar 

  3. Cohen, M. S. and Gabriele, P. D. (1982),Appl. Environ. Microbiol. 44, 23.

    CAS  Google Scholar 

  4. Cohen, M. S., Bowers, W. C, Aronson, H., and Gray, E. T., Jr. (1987),Appl. Environ. Microbiol. 53, 2840.

    CAS  Google Scholar 

  5. Cohen, M. S., Aronson, H., Feldman, K., and Gray, E. T., Jr. (1989),Proc. Thirteenth Ann. EPRI Conf. on Fuel Science and Conversion, EPRI, Palo Alto, CA, p. 9.3.

    Google Scholar 

  6. Dryden, I. G. C. (1963),Chemistry of Coal Utilization, suppl. vol. (Lowry, H. H., ed.), Wiley, New York, p. 232.

    Google Scholar 

  7. Evans, D. G. and Hooper, R. J. (1981),Coal Structure (Gorbaty, M. L. and Ouchi, K., eds.), Amer. Chem. Soc., Washington, DC, p. 195.

    Google Scholar 

  8. Faison, B. D. and Lewis, S. N. (1989),Appl. Biochem. Biotechnol. 20/21, 743.

    Google Scholar 

  9. Fakoussa, R. M. and Truper, H. G. (1983),Kolloquim in der Bergbau-Forschung Gmblt. (Rehm, H. J., ed.), Steinkohlenbergauverein, Essen, FRG, p. 41.

    Google Scholar 

  10. Gupta, R. K., Spiker, J. K., and Crawford, D. L. (1988),Can. J. Microbiol. 34, 667.

    Article  CAS  Google Scholar 

  11. Kilbane, J. J., Chatterjee, D. K., Karns, J. S., Kellogg, S. T., and Chakrabarty, A. M. (1982),Appl. Environ. Microbiol. 44, 72.

    CAS  Google Scholar 

  12. Lessie, T. G. and Gaffney, T. (1986),The Bacteria, vol X (Sokatch, J. R., ed.), Academic, New York, p. 39.

    Google Scholar 

  13. Maka, A., Srivastava, V. J., Kilbone, J. J., II, and Akin, C. (1989),Appl. Biochem. Biotechnol. 20/21, 715.

    Google Scholar 

  14. Moolick, R. T., Linden, J. C, and Karim, M. N. (1989),Appl. Biochem. Biotechnol. 20/21, 731.

    Article  Google Scholar 

  15. Nimz, H. D., Robert, O. F., and Neur, M. (1981),Holzforschung 35, 16.

    CAS  Google Scholar 

  16. Odier, E. and Rolando, C. (1985),Biochemie 67, 191.

    Article  CAS  Google Scholar 

  17. Quigley, D. R., Breckenridge, C. R., Dugan, P. R., and Ward, B.(1988),Amer. Chem. Soc. Div. Fuel Chem. Reprints 33, 580.

    CAS  Google Scholar 

  18. Quigley, D. R., Wey, J. E., Breckenridge, C. R., and Stoner, D. L. (1988),Resourc. Conserv. Recycl. 1, 163.

    Article  CAS  Google Scholar 

  19. Quigley, D. R., Ward, B., Crawford, D. L., Hatcher, H. J., and Dugan, P. R. (1989),Appl. Biochem. Biotechnol. 20/21, 753.

    Google Scholar 

  20. Palleroni, N. J. (1986),The Bacteria, vol. X (Sokatch, J. R., ed.), Academic, New York, p. 10.

    Google Scholar 

  21. Pyne, J. W., Stewert, D. L., Frederickson, J., and Wilson, B. W. (1987),Appl. Environ. Microbiol. 53, 2844.

    CAS  Google Scholar 

  22. Rosenberger, H., Scheler, G., and Kunstner, E. (1988),Fuel 67, 508.

    Article  CAS  Google Scholar 

  23. Scott, C. D., Strandberg, G. W., and Lewis, S. N. (1986),Biotechnol. Prog. 2, 131.

    CAS  Google Scholar 

  24. Strandberg, G. W. and Lewis, S. N. (1987),J. Ind. Microbiol. 1, 371.

    Article  CAS  Google Scholar 

  25. Ward, B. (1985),Syst. Appl. Microbiol. 6, 236.

    Google Scholar 

  26. Wilson, B. W., Bean, R. M., Franz, J. A., Thomas, B. L., Cohen, M. S., Aronson, H., and Gray, E. T., Jr. (1987),Energy Fuels 1, 80.

    Article  CAS  Google Scholar 

  27. Wilson, B. W., Frederickson, J., Stewert, D. L., Thomas, B. L., Campbell, J. A., and Bean, R. M. (1989),Proc. Thirteenth Ann. Conf. on Fuel Science and Conversion, EPRI. Palo Alto, CA, p. 3.3.

    Google Scholar 

  28. Wondrack, L., Szanto, M., and Wood, W. A. (1989),Appl. Biochem. Biotechnol. 20/21, 765.

    Google Scholar 

  29. Yeh, G. J. C, Ward, B., Quigley, D. R., Crawford, D. L., and Meuzalaar, H. L. C. (1988),Amer. Chem. Soc. Div. Fuel Chem. Reprints 33, 612.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Bacteriology and Biochemistry, Institute for Molecular Agricultural Genetic Engineering (IMAGE), University of Idaho, 83843, Moscow, ID

    Rajinder K. Gupta, Lee A. Deobald & Don L. Crawford

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  1. Rajinder K. Gupta
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  2. Lee A. Deobald
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  3. Don L. Crawford
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Gupta, R.K., Deobald, L.A. & Crawford, D.L. Depolymerization and chemical modification of lignite coal byPseudomonas cepacia strain DLC-07. Appl Biochem Biotechnol 24, 899–911 (1990). https://doi.org/10.1007/BF02920303

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