Abstract
Both oxidative and reductive enzymes can be utilized to enhance coal solubilization in aqueous and organic media. Aerobic solubilization was carried out with oxidases in a relatively polar medium, whereas solubilization in an anaerobic environment was conducted with reducing enzymes (dehydrogenase or hydrogenase) in aqueous and both polar and nonpolar organic media in the presence of hydrogen or a hydrogen donor. Solubilization of some enzymes in organic liquids was enhanced by complexation with polyethylene glycol. Enzyme concentration of 1–20 mg/mL was used, and most of the reactions occurred during the first 4 h, with up to 85% of the coal solubilized. There was some evidence of coal product inhibition and enzyme deactivation at higher temperatures.
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Scott, C.D., Woodward, C.A., Thompson, J.E. et al. Coal solubilization by enhanced enzyme activity in organic solvents. Appl Biochem Biotechnol 24, 799–815 (1990). https://doi.org/10.1007/BF02920296
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DOI: https://doi.org/10.1007/BF02920296