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A new Flow Injection Analysis (FIA) system was developed that permits the on-line monitoring of glucose. This information may be used to identify the biomass concentration. The proposed manifold allows the automatic analysis of glucose from 0 to 90 g/L. The results are in agreement with the analyses provided by HPLC. The system was applied to on-line monitoring of substrate in severalCandida rugosa batches grow without detecting mechanical or biochemical problems. This information was linked to a Extended Kaiman Filter (EKF) recursive estimation scheme to identify the vector state of the system.Index Entries: Flow Injection Analysis; on-line glucose analysis; biomass identification; Candida rugosa.
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Valero, F., Lafunte, J., Poch, M. et al. Biomass estimation using on-line glucose monitoring by flow injection analysis. Appl Biochem Biotechnol 24, 591–602 (1990). https://doi.org/10.1007/BF02920281
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DOI: https://doi.org/10.1007/BF02920281