Abstract
Water activation of the enzyme (an esterase of Mucor miehei) is studied during oleic acid/1-decanol esterification with or without solvent. The activation is rapid, but not instantaneous. Reaction water and water added before the beginning of the reaction do not have the same influence. The activation of the enzyme is effected by its swelling with water. When the initial quantity of water is sufficient, the reaction order is zero. But from a certain conversion, the rate decreases very suddenly or very slowly. This observation is attributed to the partitioning of an aqueous phase around the enzyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buckland, B. C., Dunnill, P., and Lilly, M. D. (1975),Biotechnol. Bioeng.17, 815–826.
Bell, G., Todd, J. R., Blain, J. A., Patterson, J. D. E., and Shaw C. E. L. (1981),Biotechnol. Bioeng.23, 1703–1719.
Zaks, A. and Klibanov, A. M. (1985),Proc. Natl. Acad. Sci. USA 82, 3192–3196.
Klibanov, A. M. (1986),Chem. Tech. 6, 354–359.
Macrae, A. R. (1985),Biocatalysts in organic syntheses, Tramper, J. Vander Plas, H. C., Linko, P., eds., Elsevier, Amsterdam, Netherlands, pp. 195–208.
Macrae, A. R. and Hammond, R. C. (1985),Biotechnol. Genet. Eng. Rev. 3, 193–217.
Funuda, T., Hirano, J., Morioka, K., Murakami, S., and Ishida, S. (1983),Nippon Kagaku Kaishi 12, 1797–1805.
Okumura, S., Iwai, M., and Tsujisaka, Y. (1979),Biochim. Biophys. Acta 575, pp. 156–165.
Gatfield, I. L., and Sand, T. (1982), Eur. Pat. Appl. EP 061023.
Gatfield, I. L. (1984),Ann. NY Acad. Sci. 434, 569–572.
Macrae, A. R. (1983),J. Amer. Oil Chem. Soc. 60, 291–294.
Boyer, J. L., Gilot, B., and Guiraud, R. (1986),Journées d’Etudes “Estérification/ Hydrolyse des Esters/Transestérification, vol.3, Toulouse, France, pp. 17–23.
Boyer, J. L., Gilot, B., and Guiraud, R. (1987),Récents Progres en Génie des Procédés, Storck A., Grévillot G., eds., Tec. Doc., Paris, France, vol4, pp. 7–14.
Boyer, J. L., Gilot, B., and Guiraud, R. (1988),XI Simposio Iberoamericano de Catalisis, Guanajuato, Mexico, June 12–17.
Zaks, A. and Klibanov, A. M. (1987),J. Biol. Chem. 263, 8017–8021.
Tai, D. F., Fu, S. L., Chuang, S. F., and Tsai, H. (1989),Biotechnol. Lett. 11, pp. 173–176.
Brink, L. E. S. and Tramper, J. (1985),Biotechnol Bioeng. 27, 1258–1269.
Reslow, M., Adlercreutz, P., and Mattiasson, B. (1987),Appl. Microbiol. Biotechnol. 26, 1–8.
Laane, C., Boeren, S., Vos, K., and Veeger, C. (1987),Biotechnol. Bioeng. 30, 81–87.
Boyer, J. L., Gilot, B., and Guiraud, R. (1989),Bull. Soc. Chim. Fr., No. 3–4, 260–264.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Boyer, J.L., Gilot, B. & Guiraud, R. Esterification with solid enzymes. Appl Biochem Biotechnol 24, 201–212 (1990). https://doi.org/10.1007/BF02920246
Issue Date:
DOI: https://doi.org/10.1007/BF02920246