Abstract
Among forty strains of marine cyanobacteria isolated in our laboratory, five strains had 1–3 different plasmids. The unicellular marine cyanobacterium,Synechococcus sp. NKBG 042902, contains at least three plasmids (pSY09, pSY10, and pSY11). However, these plasmids are cryptic. Therefore, a hybrid plasmid pUSY02 containing the 1.4kb HindIII fragment of pSY11 andEscherichia coli plasmid pUC18 was constructed. The plasmid pUSY02 transformed both marineSynechococcus sp. NKBG042902-YG1116, which is a cured strain, and fresh waterAnacystis nidulans R2 by dark incubation or Ca2+ treatment. However, the plasmid pSG111 constructed from the plasmid DNA ofA. nidulans R2 failed to transform marineSynechococcus sp. Electroporation was also applicable to transformation of marineSynechococcus sp. and fresh waterA. nidulans R2. The plasmid pUSY02 was rapidly introduced into marineSynechococcus sp.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kuhlemeier, C.J., Thomas, A. A., Van deEnde, A., Van Leen, R. W., Borrias, W. E., Van den Hondel, C. A. M. J. J., and Van Arkel, G. A. (1983),Plasmid 10, 109.
Chauvat, F., De Vries, L., Van der Ende, A., and Van Arkel, G. A. (1986),Mol. Gen. Genet. 204, 185.
Wolk, C. P., Vonshak, A., Kehoe, P., and Elhai, J. (1984),Proc. Natl. Acad. Sci. USA 81, 1561.
Shestakov, S. V. and Khyen, N. I. (1970),Mol. Gen. Genet. 107, 372.
Schmetter, G., Wolk, P., and Elhai, J. (1986),J. Bacteriol. 167, 411.
De Marsac, N. T., De la Torre, F. and Szulmajster, J. (1987),Mol. Gen. Genet. 209, 396.
Golden, S. S. and Sherman, L. A. (1983),J. Bacteriol. 155, 966.
Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. and Stainer, R. Y. (1979),J. Gen. Microbiol. 111, 1.
Chauvat, F., Astier, C., Vedel, F., and Joset-Espardellier, F. (1983),Mol. Gen. Genet. 191, 39.
Maniatis, T., Fritsch, E. T. and Sambrook, J. (1982),Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 86.
Cohen, S. N. and Chang, A. C. Y. (1973),Proc. Natl. Acad. Sci. USA 70, 1293.
Van den Hondel, C. A. M. J. J., Verbeek, S., Van der Ende, A., Weisbeek, P. J., Borrias, W. E., and Van Arkel, G. A. (1980),Proc. Natl. Acad. Sci. USA 77, 1570.
Golden, S. S. and Sherman, L. A. (1984),J. Bacteriol. 158, 36.
Chassy, B. M. and Flickinger, J. L. (1987),FEMS Microbiol. Lett. 44, 173.
Taketo, A. (1988),Biochim. Biophys. Acta 949, 318.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsunaga, T., Takeyama, H. & Nakamura, N. Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium,Synechococcus sp., and its transformation. Appl Biochem Biotechnol 24, 151–160 (1990). https://doi.org/10.1007/BF02920241
Issue Date:
DOI: https://doi.org/10.1007/BF02920241