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Production of l-malic acid via biocatalysis employing wild-type and respiratory-deficient yeasts

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Abstract

The yeastSaccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a wild-type strain and a cytoplasmic respiratory-deficient mutant devoid of functional mitochondria were employed. The mutant strain, which only contained the cytoplasmic fumarase, was still functional in fumaric acid to L-malic acid bioconversion. However, its specific conversion rate was much lower (0.20 g/g.h) than that of the wild-type strain (0.55 g/g.h).

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Authors and Affiliations

  1. Laboratory of Renewable Resources Engineering, 1295 Potter Engineering Center, Purdue University, 47907, West Lafayette, IN

    Xiaohai Wang, C. S. Gong & George T. Tsao

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  1. Xiaohai Wang
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  2. C. S. Gong
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  3. George T. Tsao
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Wang, X., Gong, C.S. & T. Tsao, G. Production of l-malic acid via biocatalysis employing wild-type and respiratory-deficient yeasts. Appl Biochem Biotechnol 70, 845–852 (1998). https://doi.org/10.1007/BF02920194

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  • DOI: https://doi.org/10.1007/BF02920194

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