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Acetamide degradation by a continuous-fed batch culture ofBacillus sphaericus

Applied Biochemistry and Biotechnology volume 70, pages 215–223 (1998)Cite this article

Abstract

The methanogenesis of acetamide occurs through a two-step reaction in methanogenic sludges. First, acetamide is hydrolyzed to acetate and ammonia by a strict aerobic bacterium(Bacillus sphaericus), then acetate is used byBacillus as carbon source or converted to methane by methanogens. In this work, the kinetics of acetamide degradation byB. sphaericus was studied in a continuous reactor with biomass accumulation, fed with acetamide. The oxygen supplied was dissolved in the feed (6.4 mg/L) to resemble conditions in an anaerobic wastewater treatment reactor. A reaction in series model (acetamide -? acetate -? biomass) was used to find the kinetic parameters. Results show thatB. sphaericus can hydrolyze acetamide in a second-order reaction withKt = 1.1 L/g/d, implying that the amount of biomass determines the rate and that no reaction will take place at specific loading rates greater than 35 gAm/gX/d. Growth parameters on acetate, as carbon source, under limiting O2 conditions, are μmax = 0.102/d,Ks = 37 mg/L, Y = 0.081 gX/gAm.

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Author notes

  1. F. Ramirez, O. Monroy, E. Favela, J. P. Guyot & F. Cruz

    Present address: ORSTOM, 911 Avenue Agropolis, BP 5045, 34032, Montpellier Cedex 1, France

Affiliations

  1. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, A P 55-535, 09340, Iztapalapa, D.F., Mexico

    F. Ramirez, O. Monroy, E. Favela, J. P. Guyot & F. Cruz

  2. ORSTOM, Mexico

    F. Ramirez, O. Monroy, E. Favela, J. P. Guyot & F. Cruz

Authors
  1. F. Ramirez
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  2. O. Monroy
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  3. E. Favela
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  4. J. P. Guyot
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  5. F. Cruz
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Ramirez, F., Monroy, O., Favela, E. et al. Acetamide degradation by a continuous-fed batch culture ofBacillus sphaericus . Appl Biochem Biotechnol 70, 215–223 (1998). https://doi.org/10.1007/BF02920138

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  • DOI: https://doi.org/10.1007/BF02920138

Index Entries

  • Acetamide
  • hydrolysis
  • Bacillus sphaericus
  • continuous culture with biomass accumulation
  • UASB reactor