Summary
In summary, we have shown that human appendix and murine PP B cells, freshly isolated from normal tissue, respond to IL-6 with significant increases in IgA SFC. Further, sIGA+ B cells from appendix express more IL-6R than is seen with B cells isolated from PBMC and spleen. When IgA subclass responses were measured, rhIL-6 induced both IgA1 and IgA2 SFC responses; however, 60–70% of the total response was represented by the IgA2 subclass. Our studies suggest that the human appendix as well as murine PP are enriched sources for sIgA+ B cells which are responsive to cytokines such as IL-6 for induction of IgA plasma cell responses.
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Kawanishi H, Saltzman L, Strober W: Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyer's patches that switch sIgM B cells to sIgA B cells in vitro. J Exp Med 1983;157:433–450.
McGhee JR, Mestecky J, Elson CO, Kiyono H: Regulation of IgA synthesis and immune response by T cells and interleukins. J Clin Immunol 1989; 9:175–199.
Murray PD, McKenzie DT, Swain SL, Kagnoff MF: Interleukin-5 and interleukin-4 produced by Peyer's patch T-cells selectively enhance immunoglobulin A expression. J Immunol 1987;139: 2669–2674.
Coffman RL, Shrader B, Carty J, Mosmann TR, Bond MW: A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization. J Immunol 1987;139:3685–3690.
Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W: The role of IL-5 in IgA B cell differentiation. J Immunol 1988;140:3033–3039.
Lebman DA, Coffman RL: The effects of IL-4 and IL-5 on the IgA response by murine Peyer's patch B cell subpopulations. J Immunol 1988;141: 2050–2056.
Matsumoto R, Matsumoto M, Mita S, Hitoshi Y, Ando M, Araki S, Yamaguchi N, Tominaga A, Takatsu K: Interleukin 5 induces maturation but not class switching of surface IgA-positive B cells into IgA-secreting cells. Immunology 1989;66:32–38.
Beagley KW, Eldrige JH, Kiyono H, Everson MP, Koopman WJ, Honjo T, McGhee JR: Recombinant murine IL-5 induces high rate IgA synthesis in cycling IgA-positive Peyer's patch B cells. J Immunol 1988;141:2035–2042.
Zilberstein A, Ruggieri A, Korn JH, Revel M: Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines. EMBO J 1986;5:2529–2537.
Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T: Purfication to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci USA 1985;82:5490–5494.
Van Snick J, Cayphas S, Szikora S, Renauld J-P, Van Roost E, Boon T, Simpson RJ: cDNA cloning of murine interleukin-HP: Homology with human interleukin 6. Eur J Immunol 1988;18:193–197.
Kishimoto T, Hirano T: Melecular regulation of B lymphocyte response. Annu Rev Immunol 1988; 6:485–512.
Kishimoto T: Review article. The biology of interleukin-6. Blood 1989;74:1–10.
Van Snick J: Interleukin-6: An overview. Annu Rev Immunol 1990;8:253–278.
Muraguchi A, Hirano T, Tang B, Matsuda T, Horii Y, Nakajima K, Kishimoto T: The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med 1988;167:332–344.
Hirata Y, Tetsuya T, Hibi M, Nakano N, Hirano T, Kishimoto T: Characterization of IL-6 receptor expression by monoclonal and polyclonal antibodies. J Immunol 1989;143:2900–2960.
Beagley KW, Eldridge JH, Lee F, Kiyono H, Everson MP, Koopman WJ, Hirano T, Kishimoto T, McGhee JR: Interleukins and IgA synthesis: Human and murine IL-6 induce high rate IgA secretion in IgA-committed B cells. J Exp Med 1989; 169:2133–2148.
Beagley KW, Edridge JH, Aicher WK, Mestecky J, Kiyono H, McGhee JR: Peyer's patch B cells with memory cell characteristics undergo terminal differentiation within 24 hours in response to IL-6. Cytokine 1991;3:107–116.
Bjerke K, Brandtzaeg P, Rognum TO: The distribution of immunoglobulin-producing cells is different in normal human appendix and colon mucosa. Gut 1986;27:667–674.
Fujihashi K, McGhee JR, Lue C, Beagley KW, Taga T, Hirano T, Kishimoto T, Mestecky J, Kiyono H: Human appendix B cells naturally express receptors for and respond to interleukin 6 with selective IgA1 and IgA2 synthesis. J Clin Invest, in press.
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McGhee, J.R., Fujihashi, K., Beagley, K.W. et al. Role of interleukin-6 in human and mouse mucosal IgA plasma cell responses. Immunol Res 10, 418–422 (1991). https://doi.org/10.1007/BF02919734
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DOI: https://doi.org/10.1007/BF02919734