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Proliferation of gastrointestinal carcinoma cells by T lymphocyte factors interleukin-3 and granulocyte-macrophage colony-stimulating factor

  • Cytokines
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Summary

Hematopoietic growth factors have been well characterized by cDNA cloning in recent years. In order to determine the influence of rhGM-CSF and rhIL-3 on epithelial cells of the gastrointestinal tract, their influence on in vitro cultured gastric and pancreas cancer cells was determined. A more than two-fold enhancement of proliferation was observed by IL-3 and GM-CSF in Mz-Sto-1 gastric and 818-4 pancreas carcinoma cells, applying a sensitive microculture system which allows precise quantification. The highest growth rates were obtained adding 1–10 ng/ml of the growth factors, but even picogram amounts were effective. Expression of mRNA for GM-CSF and IL-3 remained undetectable in the cell lines, but mRNA for the EGF receptor was demonstrated. However, IL-3 and GM-CSF did not up-regulate mRNA for the EGF receptor.

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Authors and Affiliations

  1. Department of Internal Medicine I, Johannes-Gutenberg-Universität Mainz, Langenbeckstrasse 1, D-W-6500, Mainz, (FRG)

    Wolfgang Dippold & Karl-Hermann Meyer zum Büschenfelde

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  1. Wolfgang Dippold
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  2. Karl-Hermann Meyer zum Büschenfelde
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Supported by a grant from BMFT and NMFZ.

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Dippold, W., Meyer zum Büschenfelde, KH. Proliferation of gastrointestinal carcinoma cells by T lymphocyte factors interleukin-3 and granulocyte-macrophage colony-stimulating factor. Immunol Res 10, 258–260 (1991). https://doi.org/10.1007/BF02919703

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  • DOI: https://doi.org/10.1007/BF02919703

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