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Insertal orientation has no influence on the expression ofgfp gene and the growth of the hostSynechococcus sp. PCC7942

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Abstract

In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, thegfp reporter gene was inserted in theisiAB locus ofSynechococcus sp. PCC7942 in different directions, and the GFP expression levels and the growth of the transgenic algae were compared. It was showed that thegfp gene could express in each direction, and no significant difference was detected on algal growth and GFP expression levels between the two recombinant algae.

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, P. R. China

    Lu Yongzhong & Zhang Xuecheng

  2. College of Life Science, Qingdao University of Science and Technology, 266003, Qingdao, P. R. China

    Lu Yongzhong

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  1. Lu Yongzhong
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  2. Zhang Xuecheng
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Correspondence to Zhang Xuecheng.

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Yongzhong, L., Xuecheng, Z. Insertal orientation has no influence on the expression ofgfp gene and the growth of the hostSynechococcus sp. PCC7942. J. Ocean Univ. China 5, 67–70 (2006). https://doi.org/10.1007/BF02919377

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  • DOI: https://doi.org/10.1007/BF02919377

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