Abstract
Chemical inducement and DDRT-PCR (differential display reverse transcription PCR) are adopted to investigate the effect of epinephrine (EPI) on the settlement and metamorphosis of Manila clam larvae. Chemical inducement shows that EPI has an effect to some extent on the metamorphosis of Manila clam larvae at all concentrations and in all treatments designed. The most significant result of inducement is obtained at the concentration of 10−6 mol L−1 and for 4h. DDRT-PCR using six primer pairs shows that the gene expression pattern is quite different between EPI treatment and the control. Three hundred and forty-three amplification bands are obtained in total, among which, 67 (19.53%) are differentially appeared. Therefore, EPI has an effect on the gene expression of the eye spot larval Manila clam. It can be hypothesized that EPI is a settlement and metamorphosis inducer for Manila clam. EPI may lead to larvae settlement and metamorphosis by binding to the receptors on the membrane and then changing the gene expression of larvae cells.
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References
Baloun, A. J., and D. E. Morse, 1984. Ionic control of settlement and metamorphosis in larvae Haliotis rufescens (Gastropoda).Biol. Bull.,167: 124–138.
Burke, R. D., 1983. Neural control of metamophosis in Dendraster excentrieus.Biol. Bull.,167: 176–188.
Coon, S. L., and D. B. Bonar, 1987. Pharmacological evidence that alpha adrenoreceptors mediate metamorphosic of the Pacific oyster,Crassostrea gigas. Neurosci.,23: 1169–1174.
Cris, D. J., 1974. Factors influencing the settlement of marine invertebrate larval. In:Chemoreception in Marine Organisms, Grant, P. T., and A. M. Mackie, eds., Academic Press, New York, 117–265.
Hadfield, M. G., 1984, Settlement requirements of molluscan larvae: new data on chemical and genetic roles.Aquaculture,39: 283–298.
Hong, X. G., Y. Yang, J. J. Kan, and B. L. Wu, 1998. A study on induced metamorphosis of larvae of the bay scallopArgopecten irradians.J. Oceanogr. Huanghai & Bohai Seas,16(3): 47–51.
Inestrosa, N. C., C. Gonzalez, and E. O. Campos, 1993. Metamorphosis ofConcholepas concholepas (Bruguiere, 1789) induced by excess potassium.J. Shellfish Res.,12 (2): 337–341.
Kang Q. H., J. S. Zhang, and B. H. Jin, 2003. Study on substances inducing settlement and metamorphosis of plankonic larvae ofHaliotis discus hannai.J. Fisheries Chin.,27(2): 131–136.
Ke, C. H., S. J. Li, F. X. Li, and Z. Zheng, 1998. Ionic control of attachment and metamorphosis in the green musselPerna viridis (linnaeus) larvae.Oceanologia et Limnologia Sinica,29(2): 128–134.
Ke, C. H., S. J. Li, F. X. Li, and Z. Zheng, 2000. Induction of settlement and metamorphosis ofSaccostrea cucullata larvae.J. Fisheries Chin.,24(3): 229–234.
Ke, C. H., Z. W. Sun, S. Q. Zhou, and F. X. Li, 2000. Chemical induction of settlement and metamorphosis ofChlamys nobilis.Mar. Sci.,24(12): 5–8.
Kingzett, B. C., N. Bourne, and K. Leask, 1990: Induction of metamorphosis of the Japanese scallopPatinopecten yessoensis Jay.Shellfish Res.,9(1): 119–124.
Leise, E. M., and M. G. Hadfield, 2000. An inducer of molluscan metamorphosis transorms activity patterns in a larval nervous system.Biol. Bull.,199: 241–250.
Liu, B. Z., F. S. Zhang, and Y. C. He. 1998. Study on induction of metamorphosis in larvae of bay scallop,Argopecten irradians, by some neuroactive compounds.Acta Oceanologica Sinica,20(5): 55–60.
Morse, D. E., 1990. Recent progress in larval settlement and metamorphosis: Closing the gaps between molecular biology and ecology.Bull. Mar. Sci.,46(2): 465–483.
Sambrook, J., E. F. Fritsch, and T. Maniatis, 1989.Molecular Cloning: A Laboratory Manua. Cold Spring Harbor Laboratory Press, New York, 55–56.
St John, T. P., and R. W. Davis 1979. Isolation of galactose inducible DNA sequence from saccharomyces cerevisiae by differential plaque filter hybridization.Cell,16: 443–451.
Urrutia, P. M., K. Okamoto, and N. Fusetani, 2004. Acetylcholine and serotonin induce larval metamorphosis of the Japanese short-neck clamRuditapes philippinarum.J. Shellfish Res.,23: 93–100.
Yool, A. J., S. M. Grau, M. G. Handgield, R. A. Jensem, D. A. Markell,et al., 1986. Excess potassium induces larval metamorphosis in four marine invertebrate species.Biol. Bull.,170: 255–266.
Yu, R. H., R. C. Wang, and C. Y. Tian, 2001. A study on adhering metamorphosis in eyebot larvae of bay scallopArgopecten irradians by KCl of different concentrations.Trans. Oceanol. Limnol.,4: 28–32.
Zhang, T., H. Y. Que, H. S. Yang, Y. C. He, and F. S. Zhang, 2002. Induction of metamorphosis of different day-old bay scallopArgopecten irradians by chemical cues.J. Fishery Sci. Chin.,9(3): 228–233.
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Sumin, L., Zhenmin, B., Hui, L. et al. Effect of epinephrine on the settlement and metamorphosis of Manila clam larvae. J. Ocean Univ. China 5, 141–145 (2006). https://doi.org/10.1007/BF02919213
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DOI: https://doi.org/10.1007/BF02919213