Abstract
The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0°C or 50°C for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA (Ci-ELISA) to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0°C. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50°C. In the determination of allergenicity with Ci-ELISA, a reduction in IgE binding was also observed.
Similar content being viewed by others
References
Berger, A., 2002. Science commentary: skin prick testing.British Med. J.,325 (7361): 414–429.
Besler, M., H. Steinhart, and A. Paschke, 2001. Stability of food allergens and allergenicity of processed foods.J. Chromatogr. B. Biomed. Sci. Appl.,756 (1–2): 207–228.
Bradford, M. M., 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utlilizing the principal of protein-dye binding.Anal Biochem.,72: 248–254.
Byun, M. W., and J. W. Lee, 2002. Application of gamma irradiation for inhibition of food allergy.Radia. Phys. Chem.,63: 369–370.
Daul, C. B., J. E. Morgan, and S. B. Lehrer, 1993. Hypersensitivity reactions to crustacea and mollusks.Clin. Rev. Allergy,11(2): 201–222.
Hefle, S. L., 1996. The chemistry and biology of food allergens.Food Technol.,50: 86–92.
Hirose, M., 1993. Molten globule state of food proteins.Trends Food Sci. Technol.,4: 48–51.
Laemmli, U. K., 1970. Clevage of structural proteins during the assembly of the head of bacteriophage T4.Nature,227: 680–685.
Leung, P. S., K. H. Chu, W. K. Chow, A. Ansari, C. I. Bandea,et al., 1994. Cloning, expression, and primary structure ofMetapenaeus ensis tropomyosin, the major heat-stable shrimp allergen.J. Allergy Clin. Immunol.,94: 882–890.
Li, L. H., 2003.Effects of Supersonic Wave on Mutton Tenderness. Postgraduate paper. Agricultural University of Hebei, Baoding, 38–57.
Mason, T. J., 1991.Practical Sonochemistry. Eilis Horwood Limited, England, 16–22.
Mason, T. J., 1998.Power Ultrasound in Food Processing-the Way Forward in Ultrasound in Food Processing. Thomson Science, London, 105–126.
Shimakura, K., Y. Tonomura, Y. Hamada, and K. Shiomi, 2005. Allergenicity of crustacean extractives and its reduction by protease digestion.Food Chem.,91 (2): 247–253.
Smillie, L. B., 1982. Preparation and identification of α and β tropomyosins.Method. Enzymol.,85: 234–241.
Smith, I. R., R. Cromie, and K. Stainsby, 1988. Seeing gel wells well.Anal. Biochem.,169: 370–371.
Solomon, M. B., 1997. The hydrodyne: New process to improve beef tenderness.J. Anim. Sci.,75: 1534–1537.
Suslick, K. S., 1988.Ultrasound: Its Chemical, Physical, and Biological Effects. VCH Publishers, New York, 234–248.
Towbin, H., and J. Gordon, 1984. Immunoblotting and dot blotting: current status and outlook.J. Immunol. M.,72: 313–340.
Venkatachalam, M., S. S. Tubers, K. H. Roux, and S. K. Sathe, 2002. Effects of roasting, blanching, autoclaving, and microwave heating on antigenicity of almond (Prunus dulcis L.) proteins.J. Agric. Food Chem.,50(12): 3544–3548.
Villamiel, M., E. H. Hamersveld, and D. P. Jong, 1999. Effect of ultrasound processing on the quality of dairy products.Milchwissenschaft,54(2): 69–73.
Yu, C. J., Y. F. Lin, B. L. Chiang, and L. P. Chow, 2003. Proteomics and immunological analysis of a novel shrimpollergen, Penm 2.J. Immunol.,170: 446–453.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhenxing, L., Hong, L. & Limin, C. Influence of ultrasonic treatment on the allergenic properties of Shrimp (Penaeus vannamei) Allergen. J. Ocean Univ. China 5, 115–118 (2006). https://doi.org/10.1007/BF02919208
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02919208