Biological production of ethanol from coal synthesis gas

Medium development studies

Abstract

The anaerobic bacteriaClostridium ljungdahlii produces ethanol and acetate from CO, CO2, and H2 in synthesis gas. Early studies with the bacterium showed that relatively high concentrations of ethanol could be produced by lowering the fermentation pH and eliminating yeast extract from the medium in favor of a defined medium. This article presents the results from a medium development study based on the aerobic bacteriumEscherichia coli. The results of continuous-reactor studies in a continuously stirred tank reactor (CSTR) with and without cell recycle are shown to demonstrate the utility of this improved medium.

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References

  1. 1.

    Coffin, J. M. (1984),Energy Progress 4, 134–137.

    Google Scholar 

  2. 2.

    Dashekvicz, M. P. and Uffen, R. L. (1979),J. Syst. Bacteriol. 29, 145–148.

    Article  Google Scholar 

  3. 3.

    Klasson, K. T., Lundbäck, L. M. O., Clausen, E. C., and Gaddy, J. L. (1993),J. Biotechnol. submitted for publication.

  4. 4.

    Lorowitz, W. H. and Bryant, M. P. (1984),Appl. Environ. Microbiol. 47, 961–964.

    CAS  Google Scholar 

  5. 5.

    Genthner, B. R. S., and Bryant, M. P. (1982),Appl. Environ. Microbiol. 43, 70–74.

    CAS  Google Scholar 

  6. 6.

    Grethlein, A. J., Worden, R. M., Jain, M. K., and Datta, R. (1990),Appl. Biochem. Biotechnol. 24/25, 875–884.

    Google Scholar 

  7. 7.

    Barik, S., Prieto, S., Harrison, S. B., Clausen, E. C., and Gaddy, J. L. (1988),Appl. Biochem. Biotechnol. 18, 363–378.

    CAS  Article  Google Scholar 

  8. 8.

    Vega, J. L., Prieto, S., Elmore, B. B., Clausen, E. C., and Gaddy, J. L. (1989),Appl. Biochem. Biotechnol. 20/21, 781–797.

    Google Scholar 

  9. 9.

    Clausen, E. C., Ackerson, M. D., and Gaddy, J. L. (1991), Biological Production of Ethanol from Coal, Quarterly Report 2-4-91, June–September, Contract No. DE-AC22-89PC89876.

  10. 10.

    Hungate, R. E. (1969), inMethods in Microbiology Vol. 5b, Novic, J. R. and Ribbons, D. W. eds., Academic, New York, pp. 117–132.

    Google Scholar 

  11. 11.

    Ljungdahl, L. G. and Wiegel, J. (1986), inManual of Industrial Microbiology and Biotechnology, Demain, A. L. and Solomon, N. A., eds., American Society for Microbiology, pp. 84–96.

  12. 12.

    McInerney, M. J., Bryant, M. P., and Pfennig, N. (1979),Arch. Microbiol. 122, 129–135.

    Article  CAS  Google Scholar 

  13. 13.

    Genthner, B. R. S., Davis, C. L., and Bryant, M. P. (1981),Appl. Environ. Microbiol. 42, 12–19.

    CAS  Google Scholar 

  14. 14.

    Bailey, J. E., and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill, New York, p. 28.

    Google Scholar 

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Correspondence to E. C. Clausen.

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Phillips, J.R., Klasson, K.T., Clausen, E.C. et al. Biological production of ethanol from coal synthesis gas. Appl Biochem Biotechnol 39, 559–571 (1993). https://doi.org/10.1007/BF02919018

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Index Entries

  • C. ljungdahlii
  • ethanol
  • acetate
  • synthesis gas
  • carbon monoxide
  • hydrogen