Skip to main content

Advertisement

SpringerLink
Log in

IgG autoantibodies to C1q do not detectably influence complement activation in vivo and in vitro in systemic lupus erythematosus

  • C1q Autoantibodies
  • Published:
Immunologic Research Aims and scope Submit manuscript

Abstract

The influence of IgG antibodies to C1q (C1qAb) on activation of the classical pathway of the complement system was investigated in patients with systemic lupus erythematosus (SLE). In in vivo experiments, a prototype for immune complexes was administered intravenously to 14 patients and 9 healthy controls. Eight SLE patients had increased C1qAb titers. The increase of C3a levels, which was measured as a parameter of C1 activation, was significantly lower in SLE patients than in the healthy controls (p=0.01). No correlation was found between C3a increases and C1qAb titers. In in vitro experiments the influence on C1 activation of monomeric IgG isolated from serum of 11 SLE patients, 7 of whom had increased C1qAb titers, was measured in a C4 consumption assay. The presence of C1qAb did not influence C4 consumption. The results demonstrate that C1qAb do not influence C1 activation by immune complexes in SLE patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, Suzuki K: Characterization of C1q-binding IgG complexes in systemic lupus erythematosus. Clin Immunol Immunopathol 1984;30:104–116.

    Article  PubMed  CAS  Google Scholar 

  2. Siegert CEM, Daha MR, van der Voort EAM, Breedveld FC: IgG and IgA antibodies to the collagen-like region of C1q in rheumatoid vasculitis. Arhritis Rheum 1990;33:1654–1664.

    Google Scholar 

  3. Koffler D, Agnello V, Thorburn R: Systemic lupus erythematosus: Prototype of immune complex nephritis in man. J Exp Med 1971;134(suppl):169S-179S.

    Article  PubMed  CAS  Google Scholar 

  4. Jans H, Halberg P, Lorenzen I: Circulating immune complexes in rheumatoid arthritis with extra-articular manifestations. Scand J Rheumatol 1983;12:215–218.

    Article  PubMed  CAS  Google Scholar 

  5. Tan EM, Cohen AS, Fries JF et al: The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1981; 25:1271–1277.

    Article  Google Scholar 

  6. Lobatto S, Daha MR, Breedveld FC, Pauwels EKJ, Evers-Schouten JH, Voetman AA, Cats A, van Es LA: Abnormal clearance of soluble aggregates of human immunoglobulin G in patients with systemic lupus erythematosus. Clin Exp Immunol 1988;72:55–59.

    PubMed  CAS  Google Scholar 

  7. Lobatto S, Daha MR, Voetman AA, Evers-Schouten JH, van Es AA, Pauwels EKJ, van Es LA: Clearance of soluble aggregates of human immunoglobulin G in healthy volunteers and chimpanzees. Clin Exp Immunol 1987;69:133–141.

    PubMed  CAS  Google Scholar 

  8. Daha MR, Bertina RM, Thompson J, Kauffmann RH, Nicholson-Weller A, Veltkamp JJ, Briet E: Combined hereditary deficiency of the sixth component of complement and factor VIII coagulant activity in a Dutch family. Clin Exp Immunol 1982;48:733–738.

    PubMed  CAS  Google Scholar 

  9. Doekes G, van Es LA, Daha MR: Influence of aggregate size on the binding and activation of the first. component of complement by soluble IgG aggregates. Immunology 1982;45:705–713.

    PubMed  CAS  Google Scholar 

  10. Tenner AJ, Lesavre DH, Cooper NF: Purification and radiolabeling of human C1q. J Immunol 1981; 127:648–653.

    PubMed  CAS  Google Scholar 

  11. Kolb WP, Kolb LM, Podack ER: C1q: Isolation from human serum in high yield by affinity column chromatography and development of a highly sensitive hemolytic assay. J Immunol 1979;122:2103–2110.

    PubMed  CAS  Google Scholar 

  12. Gaither TA, Alling DW, Frank MM: A new one-step method for the functional assay of the fourth component (C4) of human and guinea pig complement. J Immunol 1974;113:574–583.

    PubMed  CAS  Google Scholar 

  13. Siegert CEH, Daha MR, Westedt ML, van der Voort EAM, Breedveld FC: IgG autoantibodies against C1q are correlated with dsDNA antibodies, hypocomplementemia, and nephritis in systemic lupus erythematosus. J Rheumatol 1991;18:230–234.

    PubMed  CAS  Google Scholar 

  14. Antes U, Heinz H-P, Loos M: Evidence for the presence of autoantibodies to the collagen-like portion of C1q in systemic lupus erythematosus. Arthritis Rheum 1988;31:457–464.

    Article  PubMed  CAS  Google Scholar 

  15. Wisnieski JJ, Naff GB: Serum IgG antibodies to C1q in hypocomplementemic urticarial vasculitis syndrome. Arthritis Rheum 1989;32:1119–1127.

    Article  PubMed  CAS  Google Scholar 

  16. Schifferli JA, Yin CNg, Peters DK: The role of complement and its receptors in the elimination of immune complexes. N Engl J Med 1986;315:488–495.

    PubMed  CAS  Google Scholar 

  17. Heinz HP, Loos M: Monoclonal antibodies against components of the classical pathway of complement. Complement Inflamm 1989;6:166–174.

    PubMed  CAS  Google Scholar 

  18. Verrier Jones J, Cumming RH: Tests for circulating immune complexes; in Thompson RE (ed). Techniques in Clinical Immunology. Oxford, Blackwell, 1977, pp 136–156.

    Google Scholar 

  19. Agnello V, Koffler D, Eisenberg JW, Winchester RJ, Kunkel HG: C1q precipitins in the sera of patients with systemic lupus erythematosus and other hypocomplementemic states: Characterization of high and low molecular weight types. J Exp Med 1971;134(suppl):228S-240S.

    PubMed  CAS  Google Scholar 

  20. Robinson MF, Roberts JL, Verrier Jones J, Lewis EJ: Circulating immune complex assays in patients with lupus and membranous glomerulonephritis. Clin Immunol Immunopathol 1979;14:348–360.

    Article  PubMed  CAS  Google Scholar 

  21. Cooper KM, Moore M: Reactivity of low molecular weight material in cellular immune complex assays. Clin Exp Immunol 1983;52:407–416.

    PubMed  CAS  Google Scholar 

  22. Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, Suzuki K: Clq solid-phase radioimmunoassay: Binding properties of solid-phase C1q are evidence that C1q-binding IgG complexes in systemic lupus erythematosus are not bound to endogenous C1q. J Immunol Methods 1984;73:67–74.

    Article  PubMed  CAS  Google Scholar 

  23. Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, Suzuki K: C1q solid pahse radioimmunoassay: Evidence for the detection of antibodies directed against the collagen-like region of C1q in sera from patients with systemic lupus erythematosus Clin Exp Immunol 1987;69:98–106.

    PubMed  CAS  Google Scholar 

  24. Uwatoko S, Mannik M: Low-molecular weight C1q-binding immunoglobulin G in patients with systemic lupus erythematosus consists of autoantibodies to the collagen-like region of C1q. J Clin Invest 1988;82:816–824.

    Article  PubMed  CAS  Google Scholar 

  25. Strife CF, Leahy AE, West CD: Antibody to a cryptic, solid-phase C1q antigen in membranoproliferative nephritis. Kidney Int 1989;35:836–842.

    Article  PubMed  CAS  Google Scholar 

  26. Golan MD, Burger R, Loos M: Conformational changes in C1q after binding to immune complexes: Detection of neoantigens with monoclonal antibodies. J Immunol 1982; 129:445–447.

    PubMed  CAS  Google Scholar 

  27. Heinz HP: Biological functions of C1q expressed by conformational changes. Behring Inst Mitt 1989;84:20–31.

    PubMed  CAS  Google Scholar 

  28. Siegert CEH, Daha MR, Halma C, van der Voort EAM, Breedveld FC: IgG and IgA antibodies to C1q in systemic and renal diseases. Clin Exp Rheumatol, in press.

  29. Greisman SG, Redecha PB, Kimberley RP, Christian CL: Differences among immune complexes: Association of C1q in SLE immune complexes with renal diseases. J Immunol 1987;138:739–745.

    PubMed  CAS  Google Scholar 

  30. Jennette JC, Hipp CG: Immunohistopathologic evaluation of C1q in 800 renal biopsies. Am J Clin Pathol 1985;83:415–420.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Rheumatology, University Hospital, PO Box 9600, NL-2300 RCL, Leiden, The Netherlands

    C. E. H. Siegert, E. A. M. van der Voort & F. C. Breedveld

  2. Department of Nephrology, University Hospital, Leiden, The Netherlands

    M. R. Daha & S. Lobatto

Authors
  1. C. E. H. Siegert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. R. Daha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Lobatto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. A. M. van der Voort
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. C. Breedveld
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siegert, C.E.H., Daha, M.R., Lobatto, S. et al. IgG autoantibodies to C1q do not detectably influence complement activation in vivo and in vitro in systemic lupus erythematosus. Immunol Res 11, 91–97 (1992). https://doi.org/10.1007/BF02918613

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02918613

Key Words

Search

Navigation