Skip to main content
Log in

Biosensors based on polymer networks formed by gamma irradiation crosslinking

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Water-soluble polymers immobilized by gamma radiation have been investigated as a means of developing electrochemical sensors. Enzyme-based sensors for glucose and lactate have been made by immobilizing glucose oxidase and lactate oxidase, respectively, on platinized graphite electrodes. The enzyme is entrapped in a polymeric network of poly(vinyl alcohol) that is formed by gamma radiation crosslinking. Electrodes coated with poly (N-vinylpyrrolidone) and its corresponding monomer and then crosslinked with gamma radiation show an extraction of catecholamines into the polymer film that enhances the analytical signal for their detection by electrochemical oxidation. Poly(dimethyldiallylammonium chloride) spin-coated on a screen-printed electrochemical cell provides sufficient ionic conductivity for the cell to function as a gas sensor for oxygen, which is detected by reduction at a platinum working electrode.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. De Castro, E. S., Huber, E. W., Villarroel, D., Galiatsatos, C., Mark, J. E., Heineman, W. R., and Murray, P. T. (1987),Anal. Chem. 59, 134–139.

    Article  Google Scholar 

  2. Huber, E. W. and Heineman, W. R. (1988),Anal. Chem. 60, 2467–2472.

    Article  CAS  Google Scholar 

  3. Coury, Jr., L. A., Birch, E. M., and Heineman, W. R. (1988),Anal. Chem. 60, 553–560.

    Article  CAS  Google Scholar 

  4. Coury, Jr., L. A., Huber, E. W., Birch, E. M., and Heineman, W. R. (1989),J. Electrochem. Soc. 136, 1044–1049.

    Article  CAS  Google Scholar 

  5. Galiatsatos, C., Ikariyama, Y., Mark, J. E., and Heineman, W. R. (1990),Biosens. & Bioelect. 5, 47–61.

    Article  CAS  Google Scholar 

  6. Birch, M. E., Coury, Jr., L. A. and Heineman, W. R. (1990),Anal. Chem. 62, 1123–1130.

    Article  CAS  Google Scholar 

  7. Hajizadeh, K. (1990), Ph. D. Dissertation, University of Cincinnati.

  8. Hajizadeh, K., Halsall, H. B., and Heineman, W. R. (1991),Anal. Chim. Acta 243, 23–32.

    Article  CAS  Google Scholar 

  9. Turner, A. P. F., Karube, I., and Wilson G. S. (1987),Biosensors: Fundamentals and Applications, Oxford University Press, New York.

    Google Scholar 

  10. Imato, T., Morioka, H., and Heineman, W. R. (1992), 4th International Meeting on Chemical Sensors, Tokyo, Japan, September 13–18.

  11. Tieman, R. S., Heineman, W. R., Johnson, J., and Seguin, R. (1992),Sens. & Actuators B 8, 199–204.

    Article  Google Scholar 

  12. Tieman, R. S., Igo, D. H., Heineman, W. R., Johnson, J., and Seguin, R. (1991),Sens. & Actuators B 5, 121–127.

    Article  Google Scholar 

  13. Tieman, R. S. and Heineman, W. R. (1992),Anal. Lett. 25, 807–819.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heineman, W.R. Biosensors based on polymer networks formed by gamma irradiation crosslinking. Appl Biochem Biotechnol 41, 87–97 (1993). https://doi.org/10.1007/BF02918535

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02918535

Index Entries

Navigation