Abstract
In order to study the effect of human immunoglobulin preparations for intravenous use (IVIg) on the production and activity of interleukin-1 (IL-1) derived from monocytes, we treated cultured monocytes with IVIg and examined the lymphocyteactivating factor (LAF) activity of IL-1 in the culture supernatants. The results showed that IVIg suppressed the activity from most healthy adults and some febrile children with acute respiratory disease or Kawasaki disease. Further studies revealed that intact Ig (whole molecular Ig) did not suppress the mRNA expression of IL-1α or IL-1β in mononuclear cells, that intact Ig and pepsin-digested Ig inhibited the LAF activity of recombinant IL-1 (rIL-1) and also that intact Ig contains immunoglobulin (probably anti-IL-1 antibody) which binds with rIL-1 by dot blotting using biotin-streptavidin. These results suggest that IVIg suppresses neither IL-1 synthesis nor the release of IL-1 from monocytes but does neutralize IL-1α and IL-1β activity by binding IL-1 proteins as an anti-IL-1 antibody.
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References
Oppenheim JJ, Kovacs EJ, Matsushima K: There is more than one interleukin-1. Immunol Today 1986;7:45–56.
Okitsu-Negishi S, Dairaku M, Yamaguchi N, Furusawa S, Hashira S, Hiruma F, Ushijima H, Ito S, Yoshino K, Abe T: Production of interleukin-1 from peripheral monocytes in patients with Kawasaki disease (in Japanese). Jap J Clin Immunol 1988;11:137–147.
Rosenwasser LJ, Dinarello CA: Ability of human leukocytic pyrogen to enhance phytohemagglutinin induced murine thymocyte proliferation. Cell Immunol 1981;63:134–142.
Kumagai N, Benedict SH, Mills GB, Gelfand EW: Requirements for the simultaneous presence of phorbol esters and calcium ionophores in the expression of human T lymphocyte proliferation-related genes. J Immunol 1987;139:1393–1399.
White BA, Bancroft FC: Cytoplasmic dot hybridization. J Biol Chem 1982;257:8569–8572.
Furusawa S: Epidermal IL-1 production during DNFB-induced delayed type contact hypersensitivity (in Japanese). Teikyo Medical J 1989;12:329–341.
Iwata M, Shimozato T, Tokiwa H, Tsubura E: Antipyretic activity of a human immunoglobulin preparation for intravenous use in an experimental model of fever in rabbits. Infect Immun 1987;55:547–554.
Morgan EL, Hobbs MV, Weigle WO: Induction of IL-1 secretion from human monocytes by Fc region subfragments of human IgG1. J Immunol 1988;140:3014–3020.
Fuhlbrigge RC, Chaplin DD, Kiely JM, Unanue ER: Regulation of interleukin-1 gene expression by adherence and lipopolysaccharide. J Immunol 1987;138:3799–3802.
Warner SJC, Auger KR, Libby P: Human interleukin-1 induces interleukin-1 gene expression in human vascular smooth muscle cells. J Exp Med 1987;165:1316–1331.
Dinarello CA, Ikejima T, Warner SJC, Orencole SF, Lonnemann G, Cannon JG, Libbey P: Interleukin-1 induces interleukin-1. I. Induction of circulating interleukin-1 in rabbits in vivo and in human mononuclear cells in vitro. J Immunol 1987; 139:1902–1910.
Warner SJC, Auger KR, Libby P: Interleukin-1 induces interleukin-1. II. Recombinant human interleukin-1 induces interleukin-1 production by adult human vascular endothelial cells. J Immunol 1987;139:1911–1917.
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Okitsu-Negishi, S., Furusawa, S., Kawa, Y. et al. Suppressive effect of intravenous immunoglobulins on the activity of interleukin-1. Immunol Res 13, 49–55 (1994). https://doi.org/10.1007/BF02918224
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DOI: https://doi.org/10.1007/BF02918224