Abstract
Previous studies have shown that deficiencies of zinc and vitamin E, as well as iron excess, contribute to peroxidative damage in several tissues in vivo. The present study reports on the sensitivity of red blood cells from young rats exposed to individual or concurrent imbalances of these three nutrients. For 21 d, rats were fed diets that were either deficient or replete in zinc and with or without excess iron or replete or deficient in vitamin E. When red blood cells from these rats were incubated in vitro, erythrocyte hemolysis, lipid peroxidation (assessed by MDA production), and hemoglobin degradation (assessed by alanine release), did not significantly increase unless vitamin E had been omitted from the diet. These results imply that either adequate tightly-bound zinc exists within the zinc-deficient cell to protect it from oxidative damage, or that other antioxidant defense mechanisms (including vitamin E) present within the plasma membrane and cytosol are sufficient to protect the cell from the otherwise damaging effects of zinc deficiency and/or iron excess.
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References
L. J. Machlin and A. Bendich,FASEB J. 1, 441 (1987).
I. E. Dreosti,Family Physician,17, 684 (1988).
K. J. A. Davies and A. L. Goldberg,J. Biol. Chem. 262, 8220 (1987).
I. E. Dreosti and E. J. Partick,Biol. Trace Elem. Res. 14, 179 (1987).
W. J. Bettger and C. G. Taylor,Nutr. Res. 6, 451 (1986).
B. L. O'Dell, J. D. Browning, and P. G. Reeves,J. Nutr. 117, 1883 (1987).
M. Fontaine and V. E. O. Valli,Can. J. Comp. Med. 41, 52 (1977).
L. J. Machlin and E. Gabriel,Ann. NY Acad. Sci. 335, 98 (1980).
I. R. Record, I. E. Dreosti, R. S. Tulsi, and S. J. Manuel,Teratology 33, 311 (1986).
K. Mohri, C. Dohomoto, H. Ikesu, and O. Igarashi,Nippon Eiyo Shokuryo Gakkaishi 36, 122 (1983).
T. Asakawa and S. Matsushita,Lipids 14, 401 (1980).
L. T. Hatam and H. J. Kayden,J. Lipid Res. 20, 639 (1979).
N. W. Solomons,J. Nutr. 116, 927 (1986).
R. L. Willson,Proc. Nut. Soc. 46, 27 (1987).
S. S. Kaplay,Biochem. Med. 31, 371 (1984).
Y. S. Huang, R. Dufour, and J. Davignon,J. Am. Coll. Nutr. 2, 55 (1983).
B. L. O'Dell, J. D. Browning, and P. G. Reeves,Trace Elements in Man and Animals 5, C. F. Mills, I. Bremner, and J. K. Chesters, eds., Commonwealth Agricultural Bureaux, Slough, UK, 75 (1985).
S. J. Gross and S. A. Landaw,Ann NY Acad. Sci. 393, 315 (1982).
W. J. Bettger, P. G. Reeves, J. E. Savage, and B. L. O'Dell,Proc. Soc. Exp. Biol. Med. 163, 432 (1980).
A. J. Harding, I. E. Dreosti, and R. S. Tulsi,Nutr. Rep. Int. 36, 473 (1987).
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Record, I.R., MacQueen, S.E. & Dreosti, I.E. Zinc, iron, vitamin E, and erythrocyte stability in the rat. Biol Trace Elem Res 23, 89–96 (1989). https://doi.org/10.1007/BF02917180
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DOI: https://doi.org/10.1007/BF02917180