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Using cyclic liquid-liquid extraction method for isolation and identification of relative compounds during lignin biodegradation

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Abstract

By using the cyclic liquid-liquid extraction method the lignin biodegradated derivatives can be effectively extracted from the effluent liquors in ligninolytic enzymes treated kraft pulps. More than forty compounds were identified from the extractives by gas chromatography-mass spectrometry analysis. The result showed that lignin is treated with different ligninolytic enzymes, and the composition and content of the extractives differ obviously. These results suggest that the cyclic liquid-liquid extraction method can be used effectively as one new technique for the study of lignin biodegradation mechanisms.

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References

  1. Higuchi, T., Biosynthesis of lignin,Biosynthesis and Biodegradation of Wood Components (ed. Higuchi, T.), San Diego: Academic Press, 1985, 141–160.

    Google Scholar 

  2. Aihara, J., Why aromatic-compounds are stable,Scientific American, 1992, 266 (3): 62.

    Article  Google Scholar 

  3. Kersten, P. J., Tien, M., Kalyanaraman, B. et al., The ligninase-of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes,J. Biol. Chem., 1985, 260: 2609.

    Google Scholar 

  4. Hammel, K. E., Tien, M., Kalyanaraman, B. et al., Mechanism of oxidative Cα-Cβ cleavage of a lignin model dimer by Phanerochaete chrysosporium ligninase,J. Biol. Chem., 1985, 260: 8348.

    Google Scholar 

  5. Jefferey, F. D., Dean, J. F. D., Eriksson, K.-E.L., Biotechnological modification of lignin structure and composition in forest trees,Holzforschung, 1992, 46(2): 135.

    Google Scholar 

  6. Faix, O., Grünwald, C., Beinhoff, O., Determination of phenolic hydroxyl group content of milled wood lignins (MWL’s) from different botanical origins using selective aminolysis, FTIR,1H-NMR, and UV spectroscopy,Holzforschung, 1992, 46(2): 425.

    Google Scholar 

  7. Lu, X.-M., Li, Y.-Z., Wang, W. et al., Study of the role of lignin peroxidase produced by phanerochaete chrysosporium in depolymerization of natural lignin,Mycosystema (in Chinese), 1998, 17(2): 179.

    Google Scholar 

  8. Yamanis, J., Vilenchich, R., Addman, M., Gas-liquid chromatography of silylated glycols and terephithalate esters,J. Chromatogr., 1995, 108: 79.

    Article  Google Scholar 

  9. Pobiner, H., Improved inflection points in the non-aqueous potentiometric titration of acid functionalities in lignin chemicals by using internal standardization and ion exchange,Anal. Chim. Acta., 1983, 155: 57.

    Article  Google Scholar 

  10. Adler, E., Marton, J., Zur kenntnis der carbonylgruppen im lignin I,Acta Chem. Scand., 1959, 13: 75.

    Article  Google Scholar 

  11. Goldschmid, O., Determination of phenolic hydroxyl content of lignin preparations by ultraviolet spectrophotometry,Anal. Chem., 1954, 26(9): 1421.

    Article  Google Scholar 

  12. Månsson, P., Quantitative determination of phenolic and total hydroxyl groups in lignins,Holxforschung, 1983, 37: 143.

    Article  Google Scholar 

  13. Chen, C. L., Determination of methoxyl groups,Methods in Lignin Chemistry (eds. Lin, S. Y., Dence, C. W.), Berlin Heideberg: Springer-Verlag, 1992, 465–472.

    Google Scholar 

  14. Pew, J. C., Evidence of a biphenyl group in lignin,J. Organic Chem., 1963, 28: 1048.

    Article  Google Scholar 

  15. Stein, E. S., Estimating probabilities of correct identification from results of mass spectral library searches,J. Am. Soc. Mass. Spectrometry, 1994, 5: 316.

    Article  Google Scholar 

  16. Stein, E. S., Optimization and testing of mass spectral library search algorithms for compound identification,J. Am. Soc. Mass. Spectrometry, 1994, 5: 859.

    Article  Google Scholar 

  17. Huang Feng, Chen, J.-X., Zhan, H.-Y. et al., Bleaching bagasse kraft pulps with extrocellular enzymes of wood-rot fungi,Transactions of China Pulp and Paper (in Chinese), 1999, 14(in press).

  18. Kirk, T. K., Chang, H.-M., Decomposition of lignin by white-rot fungi. II. Characterization of heavily degraded lignin from decayed spruce wood,Holzforschung, 1975, 29: 56.

    Article  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, 250100, Jian, China

    Feng Huang & Peiji Gao

  2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, 510641, Guangzhou, China

    Jiaxiang Chen

Authors
  1. Feng Huang
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  2. Peiji Gao
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  3. Jiaxiang Chen
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Project supported by the National Natural Science Foundation of China (Grant No. 39770019).

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Huang, F., Gao, P. & Chen, J. Using cyclic liquid-liquid extraction method for isolation and identification of relative compounds during lignin biodegradation. Sci. China Ser. E-Technol. Sci. 42, 644–651 (1999). https://doi.org/10.1007/BF02917002

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  • DOI: https://doi.org/10.1007/BF02917002

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