Abstract
At physiological plasma concentrations (12–20 μM), zinc uptake by cultured human B lymphoblasts is biphasic with an early, rapid, saturable phase, followed by a slower, unsaturable phase. The saturable component is specific for zinc and cadmium, with aK m of 1–2 μM and aV max of 3.0 pmol/min/106 cells. This early phase does not require metabolic energy derived directly from ATP or the presence of sulfhydryl groups. At higher zinc concentrations (>30 μM), zinc uptake is more complex, although biphasic kinetics are also evident.
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References
A. S. Prasad,Nutr. Rev. 41, 197 (1983).
A. S. Prasad, inClinical, Biochemical, and Nutritional Aspects of Trace Elements, A. S. Prasad, ed., Alan R. Liss, New York, 1982, pp. 3–62.
M. Chvapil,Med. Clin. North Am. 60, 799 (1976).
J. K. Chesters, inClinical Biochemical, and Nutritional Aspects of Trace Elements, A. S. Prasad, ed., Alan R. Liss, New York, 1982, pp. 221–228.
P. J. Fraker,Surv. Immunol. Res. 2, 155 (1983).
K. Weismann and T. Flagstad,Acta Derm. Venerol. 56, 151 (1976).
E. Brummerstedt, A. Basse, T. Flagstad, and E. Andressen,Am. J. Pathol. 87, 725 (1977).
E. J. Moynahan,Lancet 2, 399 (1974).
J. M. Oleske, M. L. Westphal, S. Shore, D. Gorden, J. D. Bogden, and A. Nahmias,Am. J. Dis. Child. 133, 915 (1979).
L. H. Schloen, G. Fernandes, J. A. Garofalo, and R. A. Good,Clin. Bull. 9, 63 (1979).
P. J. Fraker, S. M. Haas, and R. W. Luecke,J. Nutr. 107, 1889 (1977).
G. Fernandes, M. Nair, K. Onoe, T. Tanaka, R. Floyd, and R. A. Good,Proc. Natl. Acad. Sci. USA 76, 457 (1979).
R. B. Pilz, R. C. Willis, and J. E. Seegmiller,J. Biol. Chem. 257, 13544 (1982).
J. A. Levy, D. N. Buell, C. Creech, Y. Hirshaut, and H. Silverberg,J. Natl. Cancer Inst. 46, 647 (1971).
H. L. Johnson and H. E. Sauberlich, inClinical, Biochemical, and Nutritional Aspects of Trace Elements, A. S. Prased, ed., Alan R. Liss, New York, 1982, pp. 405–426.
W. Forth and G. Nell,Agents and Actions, Suppl.8, 23 (1981).
D. R. Pfeiffer, R. W. Taylor, and H. A. Lardy,Ann. NY Acad. Sci. 307, 402 (1978).
D. R. Pfeiffer and C. M. Deber,FEBS Lett. 105, 360 (1979).
J. L. Phillips,Biochem. Biophys. Res. Commun. 72, 634 (1976).
M. L. Failla and R. J. Cousins,Biochim. Biophys. Acta 538, 435 (1978).
N. H. Stacey and C. D. Klassen,Biochim. Biophys. Acta 640, 693 (1981).
R. B. Jones, P. J. Hilton, J. Michael, J. Patrick, and V. E. Johnson,Clin. Sci. 59, 353 (1980).
M. Statter and I. Krieger,Pediatr. Res. 17, 239 (1983).
A. J. Corrigan and P. C. Huang,Biol. Trace Element Res. 3, 197 (1981).
A. J. Corrigan and P. C. Huang,Biol. Trace Element Res. 5, 25 (1983).
R. P. Cox,Mol. Pharmacol. 4, 510 (1968).
C. Ludvigsen, M. McDaniel, and P. E. Lacy,Diabetes 28, 570 (1979).
J. L. Phillips,Cell Immunol. 35, 318 (1978).
R. C. Willis and J. E. Seegmiller,Anal. Biochem. 72, 66 (1976).
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Eguchi, L.A., Willis, R.C., Seegmiller, J.E. et al. Zinc uptake by cultured human lymphoblasts. Biol Trace Elem Res 9, 177–186 (1986). https://doi.org/10.1007/BF02916525
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DOI: https://doi.org/10.1007/BF02916525