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Ascorbate oxidase

Specificity and analytical application

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Abstract

Ascorbate oxidase fromCucurbita sp. was isolated by ammonium sulfate precipitation and DEAE-dextran-silochrome column chromatography. The thermal and pH stabilities of the purified enzyme were investigated. TheK M forl-ascorbic acid (1.5 mM) and chlorohydroquinone (0.37 mM) was determined. Substrate specificity of ascorbate oxidase was investigated and compared with those of laccases fromCoriolus hirsutus andCerrena maxima. Ascorbate oxidase was covalently bound to a polymeric membrane and used in an enzyme electrode for ascorbic acid.

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References

  1. Sauberlich, H. E., Green, M. D., and Omaye, S. T. (1982), inAscorbic Acid: Chemistry, Metabolism, and Uses, Seib, P.A., ed., American Chemical Society, Washington, DC, pp. 199–222.

    Google Scholar 

  2. Matsumoto, K., Yamada, K., and Osajima, Y. (1981),Anal. Chem. 53, 1974.

    Article  CAS  Google Scholar 

  3. Esaka, M., Suzuki, K., and Kubota, K. (1985),Agric. Biol. Chem. 49, 2955.

    CAS  Google Scholar 

  4. Stevanato, R., Avigliano, L., Finazzi-Agro, A., and Rigo, A. (1985),Anal. Biochem. 149, 537.

    Article  CAS  Google Scholar 

  5. Greenway, C. (1990),Analyst 115, 1297.

    Article  CAS  Google Scholar 

  6. Ghindilis, A. L., Zhazhina, E. O., Baranov, Yu. A., Karyakin, A. A., Gavrilova, V. P., and Yaropolov, A. I. (1988),Biochemistry (Russia) 53, 735.

    Google Scholar 

  7. Lowry, O. H., Rosebrough, N. J., Farr, I. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265.

    CAS  Google Scholar 

  8. Weber, K. and Osborn, M. (1972),Meth. in Enzymol. 26, 3.

    Article  CAS  Google Scholar 

  9. Meizeraityte, M. V., Dienys, G. J., and Glemja, A. A. (1987)Biotechnology (Russia) 3, 661.

    CAS  Google Scholar 

  10. Kroneck, P. and Jakob, W. (1985),Rev. Port. Quim. 27, 47.

    Google Scholar 

  11. Tokuyama, K., Clark, E. E., and Dawson, C. R. (1965),Biochemistry 4, 1362.

    Article  CAS  Google Scholar 

  12. Nakamura, T., Makino, N., and Ogura, Y. (1968),J. Biochem. 64, 189.

    CAS  Google Scholar 

  13. Mondovi, B., Avigliano, L., Rotilio, G., Finazzi-Agro, A., Gerosa, P., and Giovagnoli, C. (1975),Mol. Cell. Biochem. 7, 131.

    Article  CAS  Google Scholar 

  14. Varfolomeyev, S. D., Naki, A., Yaropolov, A. Y., and Berezin, I. B. (1985),Biochemistry (Russia) 50, 1411.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biotechnology “Fermentas”, Graičiūno 8, 2028, Vilnius, Lithuania

    J. Sereikaité, D. Iljasevičiené & G. Dienys

  2. Academy of Agriculture, Kaunas, Lithuania

    H. Danilčenko

  3. Institute of Botany, St-Peterburgh, Russia

    V. Gavrilova

Authors
  1. J. Sereikaité
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  2. D. Iljasevičiené
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  3. G. Dienys
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  4. H. Danilčenko
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  5. V. Gavrilova
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Sereikaité, J., Iljasevičiené, D., Dienys, G. et al. Ascorbate oxidase. Appl Biochem Biotechnol 43, 153–160 (1993). https://doi.org/10.1007/BF02916440

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  • DOI: https://doi.org/10.1007/BF02916440

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