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Rapid preparation of denatured double-stranded DNA templates for sequencing

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Abstract

This article proposes protocol for rapid preparation (ds) DNA templates for sequencing based on doublestranded DNA denaturation and its recovery by extraction with Wizard™ DNA purification resin (Promega). This method is an alternative to commonly used procedure employing denatured-DNA recovery by ethanol precipitation.

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References

  1. Amersham Life Science(1995) Step-by-step protocols for DNA sequencing with Sequenase version 2.0 T7 DNA polymerase. 9th ed., Amersham, Arlington Heights, IL.

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Authors and Affiliations

  1. Department of Microbiology, University of Gdansk, ul. Kladki 24, 80-822, Gdansk, Poland

    Tadeusz Kaczorowski & Marian Sektas

  2. McArdle Laboratory for Cancer Research, University of Wisconsin, 1400 University Avenue, 53706, Madison, WI

    Tadeusz Kaczorowski & Waclaw Szybalski

Authors
  1. Tadeusz Kaczorowski
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  2. Marian Sektas
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  3. Waclaw Szybalski
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Kaczorowski, T., Sektas, M. & Szybalski, W. Rapid preparation of denatured double-stranded DNA templates for sequencing. Mol Biotechnol 11, 199–200 (1999). https://doi.org/10.1007/BF02915813

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  • DOI: https://doi.org/10.1007/BF02915813

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