Skip to main content
SpringerLink
Log in

Rabies vaccine

Developments employing molecular biology methods

  • Research
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Rabies vaccines produced by means of molecular biology are described. Recombinant vaccines employing either viruses as vectors (vaccinia, adenovirus, poxvirus, baculovirus, plant viruses) or a plasmid vector carrying the rabies virus glycoprotein gene are discussed. Synthetic peptide technology directed to rabies vaccine production is also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pérez, O. and Paolazzi, C. C. (1997) Production methods for rabies vaccine.J. Ind. Microbiol. Biotechnol. 18, 340–347.

    Article  Google Scholar 

  2. Cox, J. H., Dietzchold, B., and Schneider, L. G. (1977) Rabies viruses glycoprotein II biological and serological characterization.Infect. Immunit. 16, 754–759.

    CAS  Google Scholar 

  3. Anilionis, A., Wunner W. H., and Curtis, P. J. (1981) Structure of the glycoprotein gene in rabies virus.Nature 294, 275–278.

    Article  CAS  Google Scholar 

  4. Wiktor, T. J., Gyorgy, E., Schulmberger, H. D., Sokol, F., and Koprowski, H. (1973) Antigenic properties of rabies virus components.J. Immunol. 110, 269–276.

    CAS  Google Scholar 

  5. Sikes, R. K., Cleary, W. F., Koprowski, H., Wiktor, T. J., and Kaplan, M. M. (1977) Effective protection of monkeys against death from street virus by post-exposure administration of tissue culture rabies vaccine.Bull. W. H. O. 45, 1–11.

    Google Scholar 

  6. Flamand, A., Wiktor, T. J., and Koprowski, H. (1980) Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies related virus proteins II glycoprotein.J. Gen. Virol. 48a, 105–109.

    Article  Google Scholar 

  7. Tsiang, H. and Lagrange, P. H. (1980) In vitro detection of cell mediated immunity in street rabies infection in mice.J. Gen. Virol. 47, 183–191.

    Article  CAS  Google Scholar 

  8. Flamand, A., Wiktor, T. J., and Koprowski, H. (1980) Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies related virus proteins and the nucleocapside protein.J. Gen. Virol. 48, 97–104.

    Article  CAS  Google Scholar 

  9. Dietzschold, B., Wunner, W. H., Wiktor, T. J., Lopes, A. D., Lafon, M., Smith, C. L., and Koprowski, H. (1983) Characterization of antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus.Proc. Natl. Acad. Sci. USA 80, 70–74.

    Article  CAS  Google Scholar 

  10. Dietzschold, B., Wang, H., Rupprecht, C. E., Celis, M, Tollis, H., Herber Katz, E., and Koprowski, H. (1987) Induction of protective immunity against rabies by immunization with rabies virus ribonucle-oprotein.Proc. Natl. Acad. Sci. USA. 84, 9165–9169.

    Article  CAS  Google Scholar 

  11. Malek, L. T., Soostmeyer, G., Garvin, T., and James, E. (1984) The rabies glucoprotein gene is expressed inEscherichia coli as denatured polypeptide. Modern approaches in vaccines: molecular and chemical basis of virus virulence and immunity, Channock, R. M. and Lerner, R. A. (Eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY1, 203–208.

    Google Scholar 

  12. Old, R. W. and Primrose, S. B. (1990) Principles of genetic manipulation. Blackwell Scientific Publications 4th on line ed., Oxford University Press, Oxford, UK.

    Google Scholar 

  13. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  14. Sanger, F., Niklen, S., and Coulson, A. R. (1977) DNA sequencing with chain terminating inhibitors.Proc. Natl. Acad. Sci. USA,74, 5463–5467.

    Article  CAS  Google Scholar 

  15. Yelverton, E., Norton, S., Obijeski, J. F., and Goedel, D. (1983) Rabies virus glycoprotein analogs biosynthesis inE. coli.Science 29, 614–620.

    Article  Google Scholar 

  16. Mackett, M., Smith, G. L., and Moss, B. (1982) Vaccinia virus: A selectable eukaryotic cloning and expression vector.Proc. Natl. Acad. Sci. USA 79, 7415–7419.

    Article  CAS  Google Scholar 

  17. Prevec, L., Campbell, J. B., Christie, B. S., Belbeck, L., and Graham, F. L. (1990) A recombinant adenovirus vaccine against rabies.J. Infect. Diseases 161, 27–30.

    CAS  Google Scholar 

  18. Schneider, M., Graham, F. L., and Prevec, L. (1989) Expression of the glycoprotein of vesicular stomatitis virus by infectious adenovirus vectors.J. Gen. Virol. 70, 417–427.

    Article  CAS  Google Scholar 

  19. Graham, F. L. (1990) Adenovirus as expression vectors and recombinant vaccines.Trends Biotechnol 8, 85–87.

    Article  CAS  Google Scholar 

  20. Panicali, D. and Paoletti, E. (1982) Construction of poxvirus as cloning vectors insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.Proc. Natl. Acad. Sci. USA 79, 4927–4931.

    Article  CAS  Google Scholar 

  21. Kozak, M. (1981) Possible role of Hanking nucleotides in the recognition of the AUG initiation codon by eukaryotic ribosomes.Nucleic Acid Res. 9, 5232–5233.

    Google Scholar 

  22. Kozack, M. (1984) Point mutations close to the AUG initiation codon affect the efficiency of translation of the rat preproinsulin in vivo.Nature 308, 241–246.

    Article  Google Scholar 

  23. Kieny, M. P., Lathe, R., Dritten, R., Spehner, D., Skory, S., Schmitt, D., Wiktor, T. J., Koprowski, H., and Lecocq, J. P. (1984) Expression of rabies virus glycoprotein from a recombinant vaccinia virus.Nature 312, 163–166.

    Article  CAS  Google Scholar 

  24. Wiktor, T. J., Mac Farlan, R. J., Reagan, K. J., Dietzschold, B., Curtis, P. J., Wunner, W. H., Kieny, M. P., Lathe, R. M, Lecocq, J. P., Mackett, M., Moss, B., and Koprowski, H. (1984) Protection from rabies by vaccinia virus recombinant containing the rabies virus glycoprotein gene.Proc. Natl. Acad. Sci. USA 81, 7194–7198.

    Article  CAS  Google Scholar 

  25. Piccini, A., Perkus, M. E., and Paoletti, E. (1987) Vaccinia virus as an expression vector.Methods Enzymol. 153, 545–563.

    Article  CAS  Google Scholar 

  26. Blancou, J., Kieny, M. P., Lathe, R., Lecocq, J. P., Pastoret, P. P., Soulebot J. P., and Desmettre, P. (1986) Oral vaccination of the fox against rabies using a live recombinant vaccinia virus.Nature 322, 373.

    Article  CAS  Google Scholar 

  27. Rupprecht, Ch. E., Wiktor, T. J., Johnston, D. H., Hamir, A. N., Dietzschold, B., Wunner, W. H., Glickman, L. T., and Koprowski, H. (1986) Oral immunization and protection of raccoonsProcyon lotor with vaccinia rabies glycoprotein recombinant virus vaccine.Proc. Natl. Acad. Sci. USA 83, 7947–7950.

    Article  CAS  Google Scholar 

  28. Brochier, B., Kieny, M. P., Costy, F., Coppens, P., Bauduin, B., Lecocq, J. P., Languet, B., Chappuis, G., Desmettre, P., Afiademanyo, K., Libois, R., and Pastoret, P. P. (1991) Large scale eradication of rabies using recombinant vaccinia rabies vaccine.Nature 354, 520–522.

    Article  CAS  Google Scholar 

  29. Summer, J. W., Fekadu, M., Shaddock, J. H., Esposito J. J., and Bellini, W. J. (1991) Construction of vaccinia virus recombinants that express the rabies nucle-oprotein and protective immunization of mice.J. Virol. 183, 703–710.

    Article  Google Scholar 

  30. Fekadu, M., Summer, J. W., Shaddock, J. H., Sanderlin, D. W., and Baer, G. M. (1992) Sickness and recovery of dogs challenged with street rabies virus after vaccination with vaccinia virus recombinant expressing rabies virus N protein.J. Virol. 66, 2601–2604.

    CAS  Google Scholar 

  31. Prevec, L., Schneider, M., Rosenthal, K. L., Belbeck, L. W., Derbyshire, J. B., and Graham, F. L. (1989) Use of adenovirus based vectors for antigen expression in animals.J. Gen. Virol. 70, 429–434.

    Article  CAS  Google Scholar 

  32. Xiang, Z. Q., Yiping, Y., Wilson, J. M, and Erlt, H. C. J. (1996) A replication-defective human adenovirus recombinant serves as a highly efficacious vaccine carrier.Virology 219, 220–227.

    Article  CAS  Google Scholar 

  33. Wang, Y., Xiang, Z. Q., Pasquini, S., and Erlt, H. C. J. (1997) The use an El-deleted, replication-defective adenovirus recombinant expressing the rabies virus glycoprotein for early vaccination of mice against rabies virus.J. Virol. 71, 3677–3683.

    CAS  Google Scholar 

  34. Fries, L. F., Tartaglia, J., Taylor, J., Kauffman, E. K., Meignier, B., Paoletti, E., and Plotkin, S. (1996) Human safety and immunogenicity of canaripoxrabies glycoprotein recombinant vaccine an alternative poxvirus vector system.Vaccine 14, 428–434.

    Article  CAS  Google Scholar 

  35. Prehaud, C, Takehara, K., Flamand, A., and Bishop, D. H. L. (1989) Immunogenic and protective properties of virus glycoprotein expressed by baculovirus vectors.Virology 173, 390–399.

    Article  CAS  Google Scholar 

  36. Prehaud, C, Harris, R. D., Fulop, V., Koh, Ch. L., Wong, J., Flamand, A., and Bishop, D. H. L. (1990) Expression, characterization and purification of phos-phorylated rabies nucleoprotein synthesized in insect cells by baculovirus.Virology 178, 486–497.

    Article  CAS  Google Scholar 

  37. Matsura, Y., Possee, R., Overton, H. A., and Bishop, D. H. L. (1987) Baculovirus expression vectors, the requirements of expression proteins including glycoproteins.J. Gen. Virol. 68, 1233–1250.

    Article  Google Scholar 

  38. Fu, Z. F., Dietzschold, B., Schumacher, C. L., Wunner, W. H., Ertl, H. C. J., and Koprowski, H. (1991) Rabies virus nucleoprotein expressed in and purified from insect cells is efficacious as vaccine.Proc. Natl. Acad. Sci. USA 88, 2001–2005,

    Article  CAS  Google Scholar 

  39. Yusibov, V., Modelska, A, Steplewski, K., Agadjanyan, M., Weiner, D., Hooper, D. C, and Koprowski, H. (1997) Antigens produced in plants by infections with chimeric plant-viruses immunize against rabies virus and HIV-1.Immunology 94, 5784–5788.

    CAS  Google Scholar 

  40. Xiang, Z. Q., Spitalnik, S., Tran, M., Wunner, W. H., Cheng, J., and Erlt, H. C. J. (1994) Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus.Virology 199, 132–140.

    Article  CAS  Google Scholar 

  41. Xiang, Z. Q. and Erlt, H. C. J. (1995) Manipulation of the immune response to a plasmid encoded viral antigen by coinoculation with plasmids expressing cytokines.Immunity 2, 129–135.

    Article  CAS  Google Scholar 

  42. Crick, J. and Brown, F. (1969) Viral subunits for rabies vaccination.Nature 222, 92.

    Article  CAS  Google Scholar 

  43. Wunner, V. H., Dietzschold, B., Curtis, P. J., and Wiktor, T. J. (1983) Rabies subunit vaccines.J. Gen. Virol. 64, 1649–1656.

    Article  CAS  Google Scholar 

  44. Morein, B. and Simons, K. (1985) Subunit vaccines against enveloped viruses: virosomes, micelles and other protein immune complex.Vaccine 3, 83–93.

    Article  CAS  Google Scholar 

  45. Koprowski, H. (1988) Glimpses into the future of rabies research.Rev. Infect. Dis. 10 (suppl. 4), 810–813.

    Google Scholar 

  46. Castingolles, N., Morgeaux, S., Gontier-Jallet, C., Sarnain, D., Betbeder, D., and Perrin, P. (1996) A new family of carriers (biovectors) enhances the immunogenicity of rabies antigens.Vaccine 14, 1353–1360.

    Article  Google Scholar 

  47. Ertl, H., Varga, Y., Xiang, Z. Q., Kaiser, K., La Donna, E., and Otvos, L. (1996) Poly (DL. lactide-co-gycolide) microspheres as carriers for peptide vaccine.Vaccine 14, 879–885.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Servicio Vacuna Antirrábica, Instituto Nacional de Produccion de Biologicos, A.N.L.I.S., Av. Velez Sarsfield 563, 1281, Argentina, Buenos Aires

    Claudio Carlos Paolazzi

Authors
  1. Claudio Carlos Paolazzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Oscar Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Javier De Filippo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Claudio Carlos Paolazzi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paolazzi, C.C., Pérez, O. & De Filippo, J. Rabies vaccine. Mol Biotechnol 11, 137–147 (1999). https://doi.org/10.1007/BF02915807

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02915807

Index Entries

Search

Navigation