Abstract
This report summarizes major changes in previously published protocols for DNA extraction to improve the quality of DNA extracted from plants. Here, we highlight the critical modifications in the original protocols. The efficiency of these changes results in high-quality DNA ready to use in a variety of phytogenetically distant plant families, in particular species with mucopolysaccharides. The DNA obtained can be used without further purification in various molecular biology assays, including direct sequencing and AFLP and RAPD (random-amplified polymorphic DNA) analyses. The effectiveness of this method is proven by the amplification and sequencing of PCR products of up to 1 kb with DNA extracted from herbarium tissue ≥60 years old. This versatility is not usually found in DNA extraction protocols. In addition, this method is quick, adaptable to standard laboratories, and most important, safer and more cost-effective.
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Abbreviations
- CsCl:
-
cesium chloride
- NaCl:
-
sodium chloride
- NaOAc:
-
sodium oxaloacetate
- RAPD:
-
random amplified polymorphic DNA
- SEVAG:
-
chloroformsisoamyl alcohol
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Cota-Sánchez, J.H., Remarchuk, K. & Ubayasena, K. Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue. Plant Mol Biol Rep 24, 161–167 (2006). https://doi.org/10.1007/BF02914055
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DOI: https://doi.org/10.1007/BF02914055