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Changes in photosynthetic apparatus during dark incubation of detached leaves from control and ultraviolet-B treatedVigna seedlings

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Biologia Plantarum

Abstract

Changes in various components of photosynthetic apparatus during the 4 d dark incubation at 25°C of detached control and ultraviolet-B (UV-B) treatedVigna unguiculata L. leaves were examined. The photosynthetic apparatus was more degraded in younger control seedlings and for a longer time UV-B treated seedlings than in the older or for a shorter time UV-B treated seedlings. This was shown by determining the losses in chlorophyll (Chl) and protein contents, variable fluorescence yield, photosystem (PS) 2, PS1 and ribulose-1,5-bisphosphate carboxylase (RuBPC) activities, and photosynthetic14CO2 fixation. In contrast, the Car/Chl ratio increased during the dark incubation due to less expressed degradation of Car.

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Abbreviations

BQ:

p-benzoquinone

Chl:

chlorophyll

DCPIPH2 :

reduced 2,6-dichlorophenol indophenol

DTT:

DL-dithiothreitol

F0 :

initial fluorescence level

Fp :

peak fluorescence kevel

MV:

methyl viologen

PAR:

photosynthetically active radiation

PS:

photosystem

RuBPC:

ribulose 1,5-bisphosphate carboxylase

TCA:

trichloroacetic acid

UV-B:

ultraviolet radiation

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Authors and Affiliations

  1. School of Biological Sciences, Madurai Kamaraj University, 625 021, Madurai, India

    N. Nedunchezhian, K. C. Ravindran & G. Kulandaivelu

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  1. N. Nedunchezhian
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  2. K. C. Ravindran
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  3. G. Kulandaivelu
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Nedunchezhian, N., Ravindran, K.C. & Kulandaivelu, G. Changes in photosynthetic apparatus during dark incubation of detached leaves from control and ultraviolet-B treatedVigna seedlings. Biol Plant 37, 341–348 (1995). https://doi.org/10.1007/BF02913976

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