Abstract
Human B-lymphocyte suspension cultures of known cancer genotypes have been established from colonic mucosal biopsies taken during colonoscopy. These lines were derived from primary biopsies from patients with an autosomal dominant colon cancer syndrome (3 FPC, 1CFS, 1HCC) who previously had a colonic adenocarcinoma surgically removed but had no evidence of clinical cancer at the time of biopsy. Immunological typing demonstrated that the cells of each line were of colonic and clonal origin. All have been characterized and samples frozen as reference source.
No lymphoid suspension cultures were established from similar cultures derived from 44 other patients with and 39 clinical normals without such a colon cancer syndrome.
Such B-lymphocyte suspension cultures should provide (a) a unique source of non-senescing normal cells with known cancer genotypes growing in suspension and (b) support the concept that there is an association between the presence of B-lymphocytes and cancer gene expression in the colon wall.
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Abbreviations
- CFS:
-
Cancer Family Syndrome
- EMEM:
-
Eagle's minimum essential medium
- FBS:
-
fetal bovine serum
- FPC:
-
Familial Polyposis Coli
- GS:
-
Gardner Syndrome
- HCC:
-
Site-Specific Colon Cancer
- sc:
-
subcutaneously
References
Danes, B.S.: Epithelial line from normal human colon mucosa. J. Natl. Cancer Inst. 69, 1271–1276 (1982)
de Sousa, M. & R.A. Good: T-and B-cell populations in gut and gut-associated lymphoid organs: arrangement, migration, and function. In: Gastrointestinal Tract Cancer, Lipkin and Good, eds., Plenum Publish. pp. 29–47 (1978)
Friedman, E.A., P. J. Higgins, M. Lipkin, H. Shinya &A.M. Gelb: Tissue culture of human epithelial cells from benign colonic tumors. In Vitro 17, 632–644 (1981)
Moore, G.E., E. Ito, K. Ulrich &A.A. Sandberg: Culture of human leukemia cells. Cancer 19, 713–723 (1966)
Tomasi, T.B.Jr.: Secretory immunoglobulins. N. Engl. J. Med. 287, 500–506 (1972)
Wynder, E.L., B.S. Reddy, G.D. McCoy, J.H. Weisburger &G.M. Willians: Diet and gastro-intestinal cancer. Clin Gastroenterol. 5, 463–482 (1976)
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Danes, B.S. Heritable colon cancer syndromes: Long-term B-lymphocyte suspension cultures derived from colonic mucosal biopsies. Carlsberg Res. Commun. 49, 187–195 (1984). https://doi.org/10.1007/BF02913946
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DOI: https://doi.org/10.1007/BF02913946