Abstract
Vitellogenin-related proteins were localized within the oocytes of a marine teleost, the winter flounder, using protein A-gold immunocytochemical electron microscopy. A homologous vitellogenin antisera was used along with 15-nm protein-A gold on thin sections of oocytes. Localization was significantly confined to organelles described as yolk spheres within the oocyte cytoplasm. This technique provides specific identification of yolk-containing organelles within the developing winter flounder oocyte.
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Nagler, J.J., Murrin, F. & Idler, D.R. Localization of vitellogenin-related protein in the developing oocytes of winter flounder (Pleuronectes americanus) by protein A-gold immunocytochemistry. Cell Tissue Res 271, 567–570 (1993). https://doi.org/10.1007/BF02913741
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DOI: https://doi.org/10.1007/BF02913741