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Molecular biological markers and micrometastasis in resected non-small-cell lung cancer

Prognostic implications

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The Japanese Journal of Thoracic and Cardiovascular Surgery Aims and scope Submit manuscript

Abstract

Objectives: Recent advances in molecular biology and genetics have created new diagnostic and treatment possibilities in clinical oncology. We evaluated the usefulness of molecular biological factors in primary tumor and micrometastasis in the bone marrow and pathological negative (pN0) lymph nodes as prognostic parameters in non-small-cell lung cancer (NSCLC) patients.Methods: Pathological specimens were collected from 129 NSCLC patients to analyze molecular biological markers, including K-ras, p53, Rb, p16, loss of heterozygosity (LOH) at 3p, vascular endothelial growth factor (VEGF), and telomerase activity. Bone marrow samples from 250 NSCLC patients and pN0 lymph nodes from 85 of these patients were collected for micrometastasis detection by immunohistochemistry against cytokeratin.Results: p53 abnormalities and 3p LOH were significantly associated with reduced patient survival in adenocarcinoma, whereas VEGF expression was significantly associated with reduced survival in a squamous cell carcinoma histological subtype by univariate or multivariate analysis. We identified micrometastatic tumor cells in bone marrow of 78 (31.2%) of 250 patients and in pN0 lymph nodes of 26 (30.6%) of 85 patients. Both bone marrow and lymph nodal micrometastases wer associated with decreased survival among patients with stage I, however, only lymph nodal micrometastasis had a significant impact on survival.Conclusions: Molecular biological features of primary tumor and micrometastatic status appear useful in defining groups of patients with a poor prognosis who could benefit from adjuvant systemic treatment.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Aichi Cancer Center Hospital, Nagoya, Japan

    Tetsuya Mitsudomi

  2. Department of Surgery II, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, 807-8555, Kitakyushu, Japan

    Toshihiro Osaki, Tsunehiro Oyama, Masaaki Inoue, Chun-Dong Gu, Mantaro Kodate, Masayasu Aikawa, Tetsuya So, Makiko Mizukami & Kosei Yasumoto

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  1. Toshihiro Osaki
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  2. Tsunehiro Oyama
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  3. Masaaki Inoue
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  4. Chun-Dong Gu
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  5. Mantaro Kodate
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  6. Masayasu Aikawa
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  7. Tetsuya So
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  8. Makiko Mizukami
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  9. Tetsuya Mitsudomi
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  10. Kosei Yasumoto
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Additional information

Read at the Fifty-third Annual Meeting of The Japanese Association for Thoracic Surgery, Symposium. Oita, October 25–27, 2000.

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Osaki, T., Oyama, T., Inoue, M. et al. Molecular biological markers and micrometastasis in resected non-small-cell lung cancer. Jpn J Thorac Cardiovasc Surg 49, 545–551 (2001). https://doi.org/10.1007/BF02913530

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  • DOI: https://doi.org/10.1007/BF02913530

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